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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 778-787
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.079


Genetic Diversity and Relationships among Mango Varieties using RAPD Molecular Markers
R. Pruthvish and B.K.Chikkaswamy
Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli Hesaraghatta Road, Bangalore, India
Sigma BioScience Research Center, Indira Nagar Bangalore-560038, India
*Corresponding author
Abstract:

Mango is the genus of tree & bushy tree in the family Mangnoliaceae. Most of the Mango varieties are dioceous and cross-pollinate to produce to produce fertile hybrids suggesting a closer genetic relationship which is not expected at species level. The traditional methods using morphological characters are not successful in establishing the diversity and relationship among 19 different Mango varieties because of the environmental influence.PCR based molecular marker method, RAPD was employed to study the genetic diversity and Inter-relationship among 19 Mango varieties. On an average RAPD analysis generated 5-6 discrete bands/ varieties with 10 nucleotides primers. The size of the amplified products ranged from 100-3500 base pairs in length. With an average of 5-10 bands per primer. Of 21 amplified fragments 50 were polymorphic (80%) with at least one pair wise comparison between 19 varieties. RAPD analysis identified varieties specific amplification products which will be useful in germplasm classification and introgression studies. These results indicated that RAPD based markers are useful for genetic characterization of Mango /varieties/accessions.


Keywords: Genetic Diversity, Mango Varieties, RAPD, Molecular Markers
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 778-787. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.079