Diabetes Mellitus is a group of metabolic disorder in which there is increase in blood glucose over a prolonged period.  Out of the complications, Diabetic foot ulcer (DFU) is a major medical, social, and economic problems and is the leading cause of hospitalization for patients with diabetes. Escherichia coli, Proteus spp., Pseudomonas spp., Staphylococcus aureus and Enterococcus spp., are reported as frequent organism isolated from cases of diabetic foot infections.  Proper management of these infections requires appropriate antibiotic selection following antimicrobial susceptibility results. This study aims at evaluation of the clinical and the Microbial characteristics of diabetic foot infection in the patients in and around Kanyakumari, Tamilnadu  and the antimicrobial susceptibility pattern of the isolated bacteria.  Prevalent study to analyze the type of bacteria causing wound infection in Diabetic patients for a period of 2 months. 98 patients suffered from diabetic foot ulcer were enrolled in this study. The antimicrobial susceptibility of micro organisms is done by Kirby-Bauer disc diffusion method with the necessary discs as recommended by Central Laboratory Standard Institute.   Out of 102 isolates, 99 are found to have growth. Aerobic Gram-negative bacteria were the most commonly isolated pathogens and accounted for 94% of all isolates and gram positive which accounts for 6%. The most common individual isolates was Klebsiella pneumoniae (47%) followed by Pseudomonas aeruginosa (19%), Proteus mirabilis (15%), Citrobacter sp. (6%), Escherichia coli (6%), Acinetobacter sp. (2%), Staphylococcus aureus (3%). Klebsiella pneumoniae was more sensitive to Imipenem (89.58%) Klebsiella pneumoniae is found to be mostly sensitive to antimicrobials such as Imipenem (90%) cefoperazone sulbactum(79%), Piperacillin tazobactum (63%), chloramphenicol (60%). The organism was resistant to most of Cepalosporins, Fluoroquinolones and Co trimoxazole. proteus isolates are more sensitive to Imipenem and Cefoperazone sulbactum. It is followed by Piperacillin tazobactum (93%). Proteus is resistant to Levofloxacin (87%) and Cotrimoxazole(87%). Pseudomonas shows highest sensitivity to antimicrobials like Imipenem (89%), followed by Piperacillin tazobactum (79%), Cefoperazone-sulbactum (68%), Tobaramycin (60%) and resistance to antimicrobials such as Levofloxacin (89%), Ofloxacin (84%), Gentamicin (74%).  There is high prevalence of ESBL producers and MRSA which threatens us towards a more dreadful post antibiotic era. Application of molecular techniques may lead to more accurate microbial characterizations and targeted antibiotic therapy which is still farfetched in many tertiary care centers too. Therefore, it is necessary to evaluate the different microorganisms infecting the wound on a routine basis and to know the antibiotic susceptibility patterns of the isolates from the infected wound in patients with diabetic foot lesions which ultimately reduces the rate of amputations.

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