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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 1465-1479
DOI: https://doi.org/10.20546/ijcmas.2018.705.173


Bacteriological Spectrum and their Anti-Microbial Susceptibility Pattern in Diabetic Ulcer Patients Attending the Tertiary Care Hospital to Facilitate the Reduction in Morbidity and Amputation
Aneeta Alexis* and M. Sakthivennila
Department of Microbiology, Kanyakumari Govt Medical College, Asaripallam-629201, Kanyakumari District, India
*Corresponding author
Abstract:

Diabetes Mellitus is a group of metabolic disorder in which there is increase in blood glucose over a prolonged period.  Out of the complications, Diabetic foot ulcer (DFU) is a major medical, social, and economic problems and is the leading cause of hospitalization for patients with diabetes. Escherichia coli, Proteus spp., Pseudomonas spp., Staphylococcus aureus and Enterococcus spp., are reported as frequent organism isolated from cases of diabetic foot infections.  Proper management of these infections requires appropriate antibiotic selection following antimicrobial susceptibility results. This study aims at evaluation of the clinical and the Microbial characteristics of diabetic foot infection in the patients in and around Kanyakumari, Tamilnadu  and the antimicrobial susceptibility pattern of the isolated bacteria.  Prevalent study to analyze the type of bacteria causing wound infection in Diabetic patients for a period of 2 months. 98 patients suffered from diabetic foot ulcer were enrolled in this study. The antimicrobial susceptibility of micro organisms is done by Kirby-Bauer disc diffusion method with the necessary discs as recommended by Central Laboratory Standard Institute.   Out of 102 isolates, 99 are found to have growth. Aerobic Gram-negative bacteria were the most commonly isolated pathogens and accounted for 94% of all isolates and gram positive which accounts for 6%. The most common individual isolates was Klebsiella pneumoniae (47%) followed by Pseudomonas aeruginosa (19%), Proteus mirabilis (15%), Citrobacter sp. (6%), Escherichia coli (6%), Acinetobacter sp. (2%), Staphylococcus aureus (3%). Klebsiella pneumoniae was more sensitive to Imipenem (89.58%) Klebsiella pneumoniae is found to be mostly sensitive to antimicrobials such as Imipenem (90%) cefoperazone sulbactum(79%), Piperacillin tazobactum (63%), chloramphenicol (60%). The organism was resistant to most of Cepalosporins, Fluoroquinolones and Co trimoxazole. proteus isolates are more sensitive to Imipenem and Cefoperazone sulbactum. It is followed by Piperacillin tazobactum (93%). Proteus is resistant to Levofloxacin (87%) and Cotrimoxazole(87%). Pseudomonas shows highest sensitivity to antimicrobials like Imipenem (89%), followed by Piperacillin tazobactum (79%), Cefoperazone-sulbactum (68%), Tobaramycin (60%) and resistance to antimicrobials such as Levofloxacin (89%), Ofloxacin (84%), Gentamicin (74%).  There is high prevalence of ESBL producers and MRSA which threatens us towards a more dreadful post antibiotic era. Application of molecular techniques may lead to more accurate microbial characterizations and targeted antibiotic therapy which is still farfetched in many tertiary care centers too. Therefore, it is necessary to evaluate the different microorganisms infecting the wound on a routine basis and to know the antibiotic susceptibility patterns of the isolates from the infected wound in patients with diabetic foot lesions which ultimately reduces the rate of amputations.


Keywords: Tertiary care hospital, Diabetic ulcer, Bacteriological Spectrum
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How to cite this article:

Aneeta Alexis and Sakthivennila, M. 2018. Bacteriological Spectrum and their Anti-Microbial Susceptibility Pattern in Diabetic Ulcer Patients Attending the Tertiary Care Hospital to Facilitate the Reduction in Morbidity and Amputation.Int.J.Curr.Microbiol.App.Sci. 7(5): 1465-1479. doi: https://doi.org/10.20546/ijcmas.2018.705.173