National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacteria producing biosurfactant molecules is beneficial to manage rhizome rot of turmeric caused by Pythium spp. A detailed study was conducted on biosurfactant producing bacteria for management of rhizome rot of turmeric. Initially, a survey was conducted to find out the incidence of the disease in turmeric growing of areas of Tamil Nadu, it was observed that rhizome rot in turmeric was also ranged from 9.16 to 71.55 and the maximum incidence was noticed in Ammampalayam (71.55%). Rhizome rot affected turmeric plants were collected from various places of Tamil Nadu and used for isolation of Pythium pathogen. The bacteria were isolated from fresh roots of turmeric collected from various places of turmeric. The isolated pure culture were sent to Microbial Type Culture Collection, Institute of Microbial Technology, Chandigarh for further identification and identified as Bacillus tequilensis, Pseudomonas koreensis, and P. monteilii. Among them, P. tequilensis performed better in reducing the mycelial growth of Pythium isolates (1.18 cm) and which is followed by P. monteilii (1.19 cm). While screening of surfactant ability, B. tequilensis produced halo line at a distance of 1.5 cm in basal salt medium and 1.11 cm in Siegmund and Wagner medium amended with CTAB. Under pot culture condition, increased plant height (73.24 cm) was observed from Bacillus tequilensis when challenge inoculation with Pythium pathogen in turmeric rhizome after six month. In order to develop a bioremediation method for rhizome rot of turmeric, field trials were conducted for assessing the performance of the bacteria in the field condition against the disease and the rhizome treated with 0.2 % Bacillus tequilensis for 20 min + Soil application of B. tequilensis at 2.5 kg/ha at 3, 5 and 7 MAS was performed better in reducing the rhizome rot diseases incidence in turmeric (13.34 % at Dharmapuri and 11.67% at Gobichettipalayam). The same treatment also showed increased level of yield of rhizome over control and the yield was recorded in turmeric is 12.85 tonne/acre at Dharmapuri and 12.67 t/acre at Gobichettipalayam.
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