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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 1414-1420
DOI: https://doi.org/10.20546/ijcmas.2018.705.168


Morphological and Physiological Analysis of Bio-Control Agent (Trichoderma viride)
R.P. Mishra1, Manjul Pandey2 and Mukesh Srivastava1
1Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, India
2Department of Plant Pathology, Krishi Vigyan Kendra, Banda, India
*Corresponding author
Abstract:

Trichoderma are free-living fungi and found commonly in soil - root ecosystem. They are highly interactive in root, soil and foliar environments. They produce or release a variety of compound that induces localized or systemic resistance responses in plant. In this investigation ten different synthetic media (TSM, PDA, Asthana & Hawkers, Richard’s agar, Sabouraud’s dextrose, Rose Bengal agar, Czapex Dox, Beef extract agar, Cooke rose Bengal and Pikovaskys agar) used to study the morphological and cultural characters of the bio-control agent. Potato dextrose agar (PDA) medium supported the best growth of the bio-agent followed by Trichoderma specific medium (TSM), Pikovakyas agar and Rose Bengal medium. Beef extract agar medium supported the lowest growth of this fungus. Excellent sporulation obtained on Potato Dextrose Agar and Trichoderma Specific Medium. It was good on Pikovakyas Agar, Rose Bengal Agar and Sabouraud Agar, fair on Asthana & Hawker, Cooke Rose Bengal Agar, Czapak-dox Agar and Richard Agar, while poor on Beef Extract Agar medium. The maximum colony growth of the mycelium was observed at 250C. The statistical analysis showed that isolate T1 (8 CP) exhibited the highest growth which was significantly superior to other isolates. Rests of the isolates were at par with each other. The maximum dry weight recorded as 208.81mg, 205.80mg, 209.17mg, 207.11mg, 206.00mg, and 208.14mg at pH 7.0. The minimum dry weight recorded at pH 4.0. Thus, excellent sporulation was recorded at pH 6.5 and 7.0. It has observed that neither alkaline nor acidic conditions are congenial for the growth and sporulation of the bio-agent.


Keywords: Morphology, Sporulation, Synthetic media, pH, Temperature and T. viride
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How to cite this article:

Mishra, R.P., Manjul Pandey and Mukesh Srivastava. 2018. Morphological and Physiological Analysis of Bio-Control Agent (Trichoderma viride).Int.J.Curr.Microbiol.App.Sci. 7(5): 1414-1420. doi: https://doi.org/10.20546/ijcmas.2018.705.168