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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 1296-1300
DOI: https://doi.org/10.20546/ijcmas.2018.705.157


One Step RT-PCR Method for Quick and Reliable Detection of Citrus tristeza virus (CTV) in Mid-Hills of Meghalaya
T. Ganesh1*, T. Rajesh1, A. Banerjee2 and H. Rymbai2
1College of Post Graduate Studies, CAU, Umiam-793103, Meghalaya, India
2ICAR RC for NEH Region, Umiam-793103, Meghalaya, India
*Corresponding author
Abstract:

Citrus tristeza disease (CTD) is the most dangerous and lethal viral disease among all the viral diseases that attack to the citrus. It is caused by Citrus tristeza virus (CTV) belongs to the genus Closterovirus in the family Closteroviridae. For quick and reliable detection of CTV a one-step RT-PCR technique was standardised. The total RNA from the leaf sample was extracted by using the Quiazen RNAeasy plant mini kit. The RT-PCR kit was used for the RT-PCR reaction and the primers specific to the coat protein component of the virus is used for detection. The PCR products were subjected to the gel electrophoresis on the 1% agarose gel containing 2.5 ug of ethidium bromide (Edbr). After gel running these products are visualized under UV transilluminator. Where the CTV positive samples yield an amplicon of 672 bps but where as in negative samples there was no such amplicon. Therefore the standardized RT-PCR is found to be most sensitive and reliable method for quick detection of CTV in the infected samples.


Keywords: CTD, CTV, Closterovirus RT-PCR, Edbr
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How to cite this article:

Ganesh, T., T. Rajesh, A. Banerjee and Rymbai, H. 2018. One Step RT-PCR Method for Quick and Reliable Detection of Citrus tristeza virus (CTV) in Mid-Hills of Meghalaya.Int.J.Curr.Microbiol.App.Sci. 7(5): 1296-1300. doi: https://doi.org/10.20546/ijcmas.2018.705.157