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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 947-955
DOI: https://doi.org/10.20546/ijcmas.2018.705.117


Dynamics of Prunus Necrotic Ringspot and Apple Mosaic Ilarviruses in Stone Fruits - A Serological Perspective
Shelly Kapoor1*, Anil Handa1, Bunty Shylla2* and Abhilasha Sharma1
1Plant Virology Laboratory, Dr. Y S Parmar University of Horticulture and Forestry, Nauni-173230, Solan, Himachal Pradesh, India
2Horticulture Research and Training Station and KVK, Kandaghat, India
*Corresponding author
Abstract:

Prunus necrotic ringspot virus (PNRSV) and apple mosaic virus (ApMV) are among the most widespread and economically important ilarviruses in stone fruits. Enzyme-linked immunosorbent assay (ELISA) was used to determine the incidence of PNRSV and ApMV in peach, plum and apricot grown in the major stone fruit growing areas in the districts of Solan, Sirmour, Shimla and Kullu in Himachal Pradesh, India during active growing seasons of 2017 and 2018. Field surveys were carried out to assess the sanitary status of major stone fruits. Both ilarviruses were detected in all stone fruit species tested. Leaf samples from peach, apricot and plum were tested by ELISA for the presence of prunus necrotic ringspot virus (PNRSV) and apple mosaic virus (ApMV). The overall average infection level determined by DAS-ELISA was 11.16 percent. The most frequent virus encountered was PNRSV (9.06 percent) whereas the incidence of ApMV was 2.1 percent. This is the first large-scale study conducted on ilarviruses of stone fruits in this region of the country. Use of ELISA to monitor virus incidence in stone fruits can be put to use as an efficient and valuable tool for reducing the occurrence of ilarviruses that are transmitted by propagating material.


Keywords: Prunus necrotic ringspot virus (PNRSV), Mosaic virus (ApMV)
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How to cite this article:

Shelly Kapoor, Anil Handa, Bunty Shylla and Abhilasha Sharma. 2018. Dynamics of Prunus Necrotic Ringspot and Apple Mosaic Ilarviruses in Stone Fruits - A Serological Perspective.Int.J.Curr.Microbiol.App.Sci. 7(5): 947-955. doi: https://doi.org/10.20546/ijcmas.2018.705.117