National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Prunus necrotic ringspot virus (PNRSV) and apple mosaic virus (ApMV) are among the most widespread and economically important ilarviruses in stone fruits. Enzyme-linked immunosorbent assay (ELISA) was used to determine the incidence of PNRSV and ApMV in peach, plum and apricot grown in the major stone fruit growing areas in the districts of Solan, Sirmour, Shimla and Kullu in Himachal Pradesh, India during active growing seasons of 2017 and 2018. Field surveys were carried out to assess the sanitary status of major stone fruits. Both ilarviruses were detected in all stone fruit species tested. Leaf samples from peach, apricot and plum were tested by ELISA for the presence of prunus necrotic ringspot virus (PNRSV) and apple mosaic virus (ApMV). The overall average infection level determined by DAS-ELISA was 11.16 percent. The most frequent virus encountered was PNRSV (9.06 percent) whereas the incidence of ApMV was 2.1 percent. This is the first large-scale study conducted on ilarviruses of stone fruits in this region of the country. Use of ELISA to monitor virus incidence in stone fruits can be put to use as an efficient and valuable tool for reducing the occurrence of ilarviruses that are transmitted by propagating material.
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