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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 826-833
DOI: https://doi.org/10.20546/ijcmas.2018.705.101


Prevalence and Molecular Characterization of Scrub Typhus in Sub-Urban Regions of Vijayawada, Andhra Pradesh, India
Subhashini Nelapati*, Ch. Bindu Kiranmayi, T. Srinivasa Rao and B. Suresh
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Krishna Dist-521102, Andhra Pradesh, India
*Corresponding author
Abstract:

Scrub typhus is one of the most under-diagnosed and under-reported febrile illnesses. Hence, a study was conducted to diagnose the scrub typhus by using Weil Felix test (WF), Immuno-Chromatographic Test (ICT) and nested PCR (N-PCR). The primer pairs encoding the 56-KDa antigen were used for confirmation of Orientia tsutsugamushi. Serotype-specific primers were used for identification of Gilliam, Karp, Kato, Kawasaki and Kuroki strains. Out of the 46 blood samples collected from suspected human patients, 13 were found serologically positive by both WF and ICT and 12 by N-PCR. All the 12 N-PCR positive samples were found positive for Karp strain. None of the rat blood samples were positive for O. tsutsugamushi.


Keywords: ICT, Nested PCR, Scrub typhus and Weil Felix
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How to cite this article:

Subhashini Nelapati, Ch. Bindu Kiranmayi, T. Srinivasa Rao and Suresh, B. 2018. Prevalence and Molecular Characterization of Scrub Typhus in Sub-Urban Regions of Vijayawada, Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 7(5): 826-833. doi: https://doi.org/10.20546/ijcmas.2018.705.101