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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 743-747
DOI: https://doi.org/10.20546/ijcmas.2018.705.090


Uses of Transmission Electron Microscope in Microscopy and its Advantages and Disadvantages
Priyanka1 and Om Prakash Choudhary2*
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, Rajasthan, India
2Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl- 796015, Mizoram, India
*Corresponding author
Abstract:

Transmission electron microscopy (TEM) is a microscopic technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through. An image is formed by the interaction of the electrons transmitted through the specimen; the image is magnified and focused onto an imaging device, such as a fluorescent screen or on a layer of photographic film, or to be detected by a sensor such as a CCD camera. TEMs are capable of imaging at a significantly higher resolution than light microscopes, owing to the small de Broglie wavelength of electrons. This enables the instrument's user to examine fine detail-even as small as a single column of atoms, which is thousands of times smaller than the smallest resolvable object in a light microscope. TEM forms a major analytical tool in physical as well as biological sciences. it finds application in cancer research, virology, materials science as well as pollution, nanotechnology, and semiconductor research etc.


Keywords: TEM, Electrons, de Broglie wavelength, Nanotechnology, CCD camera
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How to cite this article:

Priyanka and Om Prakash Choudhary. 2018. Uses of Transmission Electron Microscope in Microscopy and its Advantages and Disadvantages.Int.J.Curr.Microbiol.App.Sci. 7(5): 743-747. doi: https://doi.org/10.20546/ijcmas.2018.705.090