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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 207-216
DOI: https://doi.org/10.20546/ijcmas.2018.705.027


Evaluation of Different Growth Media for Clavibacter michiganensis subsp michiganensis and Formation of Biofilm like Structures
Ruchi Tripathi*, Rashmi Tiwari and K. Vishunavat
Department of Plant Pathology, College of Agriculture, G B Pant University of Ag and Technology, India
*Corresponding author
Abstract:

The studies to work out the most suitable medium for the growth of Clavibacter michiganensis subsp michiganensis was carried out using nonselective (NA, NGY, YPGA, YDCA and SPY) and semi-selective media (SCM, D2ANX). Besides the systemic nature of the pathogen was assessed through formation of biofilm like structures in xylem sap (XS), minimal medium, LB broth and in distills water under in vitro condition. Amongst the tested nonselective medium NGY was observed to be the most suitable medium for growth with a growth area ranging from 213-232 mm2 (4th DAP) and 1389-1412 mm2 (10th DAP). Amongst the semi- selective media, D2ANX was observed to be the most effectual for the bacterial growth with a growth area ranging from 26- 58 mm2 and 32-61 mm2 (10th DAP). Although, NGY medium was found to be the most suitable for the growth of the bacterium Cmm but at the same time it preferred the growth of bacteria other than Cmm whereas on D2ANX and SCM a slow growth of Cmm was observed yet these media did not support the growth of bacteria other than Cmm. For the formation of biofilm like structure, the maximum aggregates formation of the pathogen was observed in xylem sap by five Cmm isolates with and OD value ranging between of 0.546- 0.162, the least being in case of Cmm 1 isolate.


Keywords: Tomatoes, Cmm, Growth medium, Biofilm like structures formation, Selectivity
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How to cite this article:

Ruchi Tripathi, Rashmi Tiwari and Vishunavat, K. 2018. Evaluation of Different Growth Media for Clavibacter michiganensis subsp michiganensis and Formation of Biofilm like Structures.Int.J.Curr.Microbiol.App.Sci. 7(5): 207-216. doi: https://doi.org/10.20546/ijcmas.2018.705.027