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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 80-82
DOI: https://doi.org/10.20546/ijcmas.2018.705.011


Standardization of Primary Hepatic Cell Culture in Chicken
Athe Rajendra Prasad1*, T.K. Bhattacharya1, R.N. Chatterjee1, P. Guruvishnu2 and N. Govardhana Sagar3
1ICAR-Directorate of Poultry Research, Rajendra nagar, Hyderabad, Telangana, India
2Sri Venkateswara Veterinary University, Tirupathi, AP, India
3ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, India
*Corresponding author
Abstract:

Liver is one of the important organs in the body that regulates various metabolisms in the body and having multiple functions. It is difficult to study all the functions of the liver in vivo, because of other organs interventions. Hence, the liver parenchymal cells (hepatocytes) were cultured in vitro in order to study the different activities of the liver. In the present study, we standardized culturing of chicken embryo hepatocytes from 12 to 14 days old embryos. Here we have used 0.125% trypsin EDTA to disaggregate the parenchymal cells of the liver. The cells were cultured using growth media added with 10% Fetal Bovine Serum and Tryptone Phosphate Broth.


Keywords: Primary hepatic cell culture, Chicken
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How to cite this article:

Athe Rajendra Prasad, T.K. Bhattacharya, R.N. Chatterjee, P. Guruvishnu and Govardhana Sagar, N. 2018. Standardization of Primary Hepatic Cell Culture in Chicken.Int.J.Curr.Microbiol.App.Sci. 7(5): 80-82. doi: https://doi.org/10.20546/ijcmas.2018.705.011