Standardization of Primary Hepatic Cell Culture in Chicken

Athe Rajendra Prasad; T.K. Bhattacharya; R.N. Chatterjee; P. Guruvishnu; N. Govardhana Sagar

doi:https://doi.org/10.20546/ijcmas.2018.705.011

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Original Research Articles Volume : 7, Issue:5, May, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2018.7(5): 80-82

DOI: https://doi.org/10.20546/ijcmas.2018.705.011

Standardization of Primary Hepatic Cell Culture in Chicken

Athe Rajendra Prasad^1*, T.K. Bhattacharya¹, R.N. Chatterjee¹, P. Guruvishnu² and N. Govardhana Sagar³

¹ICAR-Directorate of Poultry Research, Rajendra nagar, Hyderabad, Telangana, India
²Sri Venkateswara Veterinary University, Tirupathi, AP, India
³ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, India

^*Corresponding author

Abstract:

Liver is one of the important organs in the body that regulates various metabolisms in the body and having multiple functions. It is difficult to study all the functions of the liver in vivo, because of other organs interventions. Hence, the liver parenchymal cells (hepatocytes) were cultured in vitro in order to study the different activities of the liver. In the present study, we standardized culturing of chicken embryo hepatocytes from 12 to 14 days old embryos. Here we have used 0.125% trypsin EDTA to disaggregate the parenchymal cells of the liver. The cells were cultured using growth media added with 10% Fetal Bovine Serum and Tryptone Phosphate Broth.

Keywords: Primary hepatic cell culture, Chicken

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How to cite this article:

Athe Rajendra Prasad, T.K. Bhattacharya, R.N. Chatterjee, P. Guruvishnu and Govardhana Sagar, N. 2018. Standardization of Primary Hepatic Cell Culture in Chicken.Int.J.Curr.Microbiol.App.Sci. 7(5): 80-82. doi: https://doi.org/10.20546/ijcmas.2018.705.011

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 7, Issue:5, May, 2018

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