Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 15-23
DOI: https://doi.org/10.20546/ijcmas.2018.705.003


Success in Inducing Effective Rooting in Triticale x Wheat and Wheat x Wheat Derived Haploid Plantlets in Cocopeat Mixture
Navdeep S. Jamwal1*, Harinder K. Chaudhary1, Anila Badiyal1, Waseem Hussain2, Pankaj Katoch1 and Nimit Kumar1
1Molecular Cytogenetics and Tissue Culture Lab., Department of Crop Improvement, CSK HP Agricultural University, Palampur-176062, India
2Department of Agronomy and Horticulture, University of Nebraska Lincoln-68588-0915, USA
*Corresponding author
Abstract:

Liquid Murashige and Skoog (MS) medium in varying strengths, is the most commonly utilized medium for proficient rooting in the regenerated plants in doubled haploidy breeding protocols. On the other side, CPV (cocopeat: perlite: vermiculite) medium is currently being used in hardening of tissue culture regenerated plants. Hence, the present study was designed to check the relative efficacy of both media for the development of various root characteristics in triticale and wheat derived haploid plantlets. Significant difference for root traits was observed in both media revealing higher average root length of the plantlets (22.04cm) in CPV than that of half strength liquid MS medium (14.54cm). Further, average root surface area increased almost four times in CPV (21.14cm2) than liquid medium (5.53cm2). Similarly mean number of roots was also found to be more (seven) in CPV than liquid medium (five). The survival rate of haploids after colchicine application from liquid medium was observed to be far less than in CPV. Conclusively, CPV medium was observed as more efficient alternative to liquid medium for high recovery of doubled haploids.


Keywords: Triticale, Wheat, Haploid, Liquid MS medium, Cocopeat, Perlite, Vermiculite, Roots
Download this article as Download

How to cite this article:

Navdeep S. Jamwal, Harinder K. Chaudhary, Anila Badiyal, Waseem Hussain, Pankaj Katoch and Nimit Kumar. 2018. Success in Inducing Effective Rooting in Triticale x Wheat and Wheat x Wheat Derived Haploid Plantlets in Cocopeat Mixture.Int.J.Curr.Microbiol.App.Sci. 7(5): 15-23. doi: https://doi.org/10.20546/ijcmas.2018.705.003