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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 3705-3710
DOI: https://doi.org/10.20546/ijcmas.2018.704.416


In vitro Estimation on the Effect of Imidacloprid on the Growth and Multiplication Rate of Rhizobium for a Strategic- Ecofriendly Seed Treatment in Pulse
M. Gnanachitra1*, V.K. Satya2 and P. Akiladevi3
1Department of Agriculture Microbiology
3Department of Plant Pathology, National Pulses Research Centre, TamilNadu Agricultural University, Vamban, Pudukottai-622 303, Tamil Nadu, India
2Department of Plant Pathology, KVK, Tindivanam, Tamil Nadu, India
*Corresponding author
Abstract:

In Pulses particularly in blackgram and greengram seed treatment, Imidacloprid is one of the important component used for the control of viral diseases particularly Yellow Mosaic disease, in addition, biofertilizers viz., Rhizobium and phosphobacteria also necessarily used to promote nodulation and nitrogen fixation. These field recommendations should be well supported by in vitro assays and testing the compatibility of the pesticides with the biofertilizers and biopesticides used for seed treatment. To confirm the compatibility an in vitro study was conducted on the effect of Imidacloprid dosage on the growth and multiplication using YEMA broth and the growth rate was enumerated in Spectrophotometer. Imidacloprid did not prevent the growth of Rhizobium in the growth media rather Imidacloprid showed a retarded effect towards Rhizobium hence, the growth of Rhizobium was initially set back and registered very slow growth rate upto 1½ days after that it attained normal growth and reached its log phase.


Keywords: Pulses, Blackgram, Seed treatment, Rhizobium, Imidacloprid
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How to cite this article:

Gnanachitra, M., V.K. Satya and Akiladevi, P. 2018. In vitro Estimation on the Effect of Imidacloprid on the Growth and Multiplication Rate of Rhizobium for a Strategic-Ecofriendly Seed Treatment in Pulse.Int.J.Curr.Microbiol.App.Sci. 7(4): 3705-3710. doi: https://doi.org/10.20546/ijcmas.2018.704.416