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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 3361-3372
DOI: https://doi.org/10.20546/ijcmas.2018.704.380


Isolation of Biocidal Compounds of Calotropis Latex and their in vitro and in vivo Effect on the Mortality of Root Knot Nematode (Meloidogyne javanica) in Brinjal and Chilli
Lalit Kumar1*, Abhilasha Srivastava2, Bansa Singh1 and A.P. Khare1
1Indian Institute of Pulses Research, Kanpur, India
2(WOS-B), Department of Science and Technology, New Delhi, India
*Corresponding author
Abstract:

An attempt was made to find out natural and cheaper methods for the control of vegetable pest. Locally available plant C. procera was used and a good methodology was developed to extract the secondary metabolic compounds from the latex. Secondary metabolic compounds extracted in form of different polarity fractions and in purity of >90% as single pure compound in each fraction, showed tremendous activity in vitro test against secondary stage juveniles of Meloidogyne javanica. The extracted fractions were found toxic to juveniles by different capabilities between concentrations ranged from 50-300 g ml-1. The activity of compounds was found dependent on concentration, time of exposure and the polarity of the extracted fractions. Two highly lipophilic nature of fractions having clear solubility in solvents such as hexane, dichloromethane were found extremely active by giving larval mortality of test nematode by more than 90% at their 250 g ml-1 concentrations, after an exposure period of 48hr. whereas, quick and absolute mortality in these fractions was observed at 300 g ml-1 concentrations. Ethyl acetate soluble fraction also gave more than 80% mortality of test nematodes at 250 and 300 g ml-1 concentrations, after an exposure period of 48hr. A high concentration viz., 1000 g ml-1 of the fraction especially derived from polar part of latex and found soluble in methanol was required to bring about 80% juvenile mortality of the test nematode that to after an exposure period of 72hrs. The EC formulations developed from non-polar fractions during their bio-efficacy test in pots also revealed a good control of nematode gall formation on roots of brinjal and chilli which on turn found to enhance yield of both of the crops to a great extent. As compare to control, the three formulations especially developed from hexane, dichloromethane and ethyl acetate soluble fractions were found to control nematode galls on root nearly to 50% on brinjal and 40% on chilli at their concentrations of 1.25g/pot. The formulations at this concentration also gave a yield enhancement of 70-100% and 50-60% respectively in case of brinjal and chilli as compare to control.


Keywords: Root knot nematode (Melidogyne javanica)
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How to cite this article:

Lalit Kumar, Abhilasha Srivastava, Bansa Singh and Khare, A.P. 2018. Isolation of Biocidal Compounds of Calotropis Latex and their in vitro and in vivo Effect on the Mortality of Root Knot Nematode (Meloidogyne javanica) in Brinjal and Chilli.Int.J.Curr.Microbiol.App.Sci. 7(4): 3361-3372. doi: https://doi.org/10.20546/ijcmas.2018.704.380