National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
V. jatamansi Jones is an important medicinal wild herb of North Eastern Himalayan Region belonging to family Valerianaceae. The herb is widely used in treatment of leprosy, epilepsy, hysteria and asthma and also in making perfumed powder. Due to over-exploitation of roots and rhizomes, V. jatamansi is included in endangered species as per National Medicinal Plant Board, New Delhi, India. The harvest of roots and rhizomes causes destruction of whole plant at its natural habitat. An alternative and efficient protocol was developed for in vitro root mass multiplication from leaf explant in order to promote in situ conservation of the herb. The leaf explant sterilized with 5% (v/v) Tween-20 (5 min), 0.1% Bavistin (5 min), 0.1% (w/v) HgCl2 (1 min) and 70% ethanol (1 min) and exhibited 80% survival after 7 days of implantation on MS medium supplemented with 2 mg/L each of BAP and NAA. However, 90 % of leaf explant in same medium induced roots exhibiting maximum root number (70) and maximum root length (5 cm.).
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