Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 3108-3114
DOI: https://doi.org/10.20546/ijcmas.2018.704.352


In vitro Direct Rhizogenesis of an Endangered Medicinal Herb: Valeriana jatamansi Jones
Soniya Saini1, Ashutosh Dubey1, Atul Kumar2, Gohar Taj3, Soumya Budhori1 and Vandana A. Kumar1*
1Department of Biochemistry
2Department of Plant Physiology,
3Department of MBGE, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, India
*Corresponding author
Abstract:

V. jatamansi Jones is an important medicinal wild herb of North Eastern Himalayan Region belonging to family Valerianaceae. The herb is widely used in treatment of leprosy, epilepsy, hysteria and asthma and also in making perfumed powder. Due to over-exploitation of roots and rhizomes, V. jatamansi is included in endangered species as per National Medicinal Plant Board, New Delhi, India. The harvest of roots and rhizomes causes destruction of whole plant at its natural habitat. An alternative and efficient protocol was developed for in vitro root mass multiplication from leaf explant in order to promote in situ conservation of the herb. The leaf explant sterilized with 5% (v/v) Tween-20 (5 min), 0.1% Bavistin (5 min), 0.1% (w/v) HgCl2 (1 min) and 70% ethanol (1 min) and exhibited 80% survival after 7 days of implantation on MS medium supplemented with 2 mg/L each of BAP and NAA. However, 90 % of leaf explant in same medium induced roots exhibiting maximum root number (70) and maximum root length (5 cm.).


Keywords: Valeriana jatamansi, Explant, Rhizogenesis, MS medium
Download this article as Download

How to cite this article:

Soniya Saini, Ashutosh Dubey, Atul Kumar, Gohar Taj, Soumya Budhori and Vandana A. Kumar. 2018. In vitro Direct Rhizogenesis of an Endangered Medicinal Herb: Valeriana jatamansi Jones.Int.J.Curr.Microbiol.App.Sci. 7(4): 3108-3114. doi: https://doi.org/10.20546/ijcmas.2018.704.352