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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2947-2960
DOI: https://doi.org/10.20546/ijcmas.2018.704.335


Study of the Antimicrobial Effect of the Different Combinations of Silver Nano-Particles, Antibiotics, Laser and Photo-Sensitizer on Gram Positive and Gram Negative Bacteria
Dalia El-Sayed Metwally1*, Eglal Abd El-Salam El-Sherbiny1, Heba Saed Ramadan2, Moustafa Moustafa Mohamed Ahmed2 and Mo,men Gameel Abd Elmageed3
1Department of Microbiology, Medical Research Institute, University of Alexandria, Egypt
2Department of Medical Biophysics, Medical Research Institute, University of Alexandria, Egypt
3Faculty of Science, Sohag University, Egypt
*Corresponding author
Abstract:

Antimicrobial resistance is one of the major threats to human health. Thereby, discovering alternative strategies that can circumvent the mechanisms of resistance presents a big challenge. One of which is the use of nanotechnology and Photodynamic therapy. The current study included a total of 40 isolates that were; Twenty isolates of Staphylococcus aureus (14 MRSA and 6 MSSA isolates) and twenty isolates of multiple drug resistance (MDR) Gram negative bacteria (4 Escherichia coli, 4 Pseudomonas spp., 6 Klebsiella spp, 4 Proteus spp., 1 Acinetobacter spp. and one Enterobacter spp. isolates.). The effects due the interaction of silver nanoparticles (SNPs), laser in the presence of antibiotics (Polymyxin B for Gram negative bacteria and Fusidic acid for Gram positive bacteria) and photo-sensitizer; methylene blue (MB) on the test isolates was investigated by micro dilution test. The combinations that showed a statistically significant change in MIC values were those with the different combinations containing SNP. The effect of these combinations on the inhibition of growth of the Gram positive and/ or Gram negative isolates showed a statistically significant difference. In vivo studies are needed to determine whether considerable bacterial kills can be obtained in a wound model.


Keywords: Staphylococcus aureus, Enterobacter spp. isolates
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How to cite this article:

Dalia El-Sayed Metwally, Eglal Abd El-Salam El-Sherbiny, Heba Saed Ramadan, Moustafa Moustafa Mohamed Ahmed and Mo,men Gameel Abd Elmageed. 2018. Study of the Antimicrobial Effect of the Different Combinations of Silver Nano-Particles, Antibiotics, Laser and Photo-Sensitizer on Gram Positive and Gram Negative Bacteria.Int.J.Curr.Microbiol.App.Sci. 7(4): 2947-2960. doi: https://doi.org/10.20546/ijcmas.2018.704.335