Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2924-2931
DOI: https://doi.org/10.20546/ijcmas.2018.704.333


Detection of Cryptosporidium species among Malnourished Children in and around Chennai, Tamil Nadu, Southern India
G.S. Chamundeeswari*, R. Raghuraman and M. Raman
Department of Microbiology, Stanley Medical College, Chennai 600 001, Tamilnadu, India
*Corresponding author
Abstract:

Aim of the study is to detect Cryptosporidium, a newly emerging parasite in malnourished children with and without diarrhea, using conventional methods and special staining techniques. A total of 727 children comprised of 497 malnourished and 230 normal children having with and without diarrhoea. Two consecutive samples were collected from each individual and one preserved using 10% formalin. Examination of helminthes and protozoan in the stool samples were done by direct wet mount method and by formalin ether sedimentation method. Smear made from the deposit were stained using modified Kinyoun’s Acid Fast stain for Cryptosporidium. The size of the cryptosporidium was measured using micrometer. Among 727 samples, 12/727 (1.65%) were found to be positive for Cryptosporidium. All the individuals were found to have profuse watery diarrhea. Other enteric parasites such as Giardia, Hookworm, Entamoeba histolytica were also detected. Children within the age group of >5 year were found to be more vulnerable for Cryptosporidial infection.


Keywords: Cryptosporidium, Diarrhea, Malnourished children
Download this article as Download

How to cite this article:

Chamundeeswari, G.S., R. Raghuraman and Raman, M. 2018. Detection of Cryptosporidium species among Malnourished Children in and around Chennai, Tamil Nadu, Southern India.Int.J.Curr.Microbiol.App.Sci. 7(4): 2924-2931. doi: https://doi.org/10.20546/ijcmas.2018.704.333