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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 204-213
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.022


Identification of Prostate Specific Symbiont in Earthworm (Eudrilus eugeniae) and its Role in Reproduction
Divya S. Raj, Bency Rachel, H. Sridhar and S. Umamaheswari*
Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli- 627 012
*Corresponding author
Abstract:

Earthworms have been found enormously with the presence of symbionts in various organs. The earthworm, Eudrilus eugeniae is harbouring symbionts in the prostate gland. The molecular analysis of 16SrRNA exposed the presence of two bacterial isolates in the prostate gland, one specific for the prostate gland and the other one common harbouring in the clitellum was found out. Gram negative, rod shaped, Catalase negative and Oxidase positive bacterium was identified to be specific for the prostate gland. 16S rRNA gene sequences of the prostate specific isolate indicated its relatedness to the genus Enterobacteriaeceae. Gene sequence assembled and phylogenetic analysis revealed the isolate with 99% similarity with Enterobacter sp. The isolate harboured in the clitellum and prostate indicated its relatedness to Bacillus cereus. Both the bacterial isolates were found to be present in the entire life stages of earthworm. Predominance of the bacterium was found in the stage of cocoon, with least presence at the juvenile stage. Experimental studies explored the importance of prostate specific isolate associated with the fecundity rate.


Keywords: Eudrilus eugeniae,16S rRNA gene sequences,Enterobacter sp.Gene sequence,prostate gland
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How to cite this article:

Divya S. Raj, Bency Rachel, H. Sridhar and S. Umamaheswari. 2016. Identification of Prostate Specific Symbiont in Earthworm (Eudrilus eugeniae) and its Role in Reproduction.Int.J.Curr.Microbiol.App.Sci. 5(8): 204-213. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.022