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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2552-2558
DOI: https://doi.org/10.20546/ijcmas.2018.704.292


Assessment of Clonal Fidelity and Genetic Analysis of Tissue Culture Raised Banana (Musa spp.) Plant
Jotshana Manik Maske1*, Amol Dadarao Sable1 and Prashant Bhausaheb Kardile2
1Department of Plant Biotechnology, SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.)-431001, India
2Department of Agricultural Botany, Dadasaheb Patil College of Agriculture, Dahegaon, Auranagabad (M.S.)-423715, India
*Corresponding author
Abstract:

Banana (Musa spp.) is important fruit crop in India. Banana is one of the oldest and most popular fruit grown in India with great socio-economic significance. The present study aims to assessment of clonal fidelity and genetic analysis of tissue culture raised banana (Musa spp.) plant. Banana tissue culture propagation is a new an established a popular technology due to the advantages. Through plant tissue culture method large scale production is possible in short time. The most reliable methods are the molecular marker techniques that identify the variance depending on the plant proteins, which are expressed from defined regions of DNA, or DNA polymorphisms. RAPD (random amplified polymorphic DNA’s) is a powerful technique for identification of genetic variation (Welsh et al., 1990). The RAPD technique is effective to develop genotype-specific banding patterns valuable for cultivar identification. Molecular analysis using RAPD’s can be applied to assess the clonal fidelity of plants derived in-vitro on an industrial scale as part of crop improvement programs. With the primer OPA-19 and OPC-1 amplified 1 mother and 4 daughter subculture are showed polymorphic bands is seen range of 500bp to 1000pb.


Keywords: Banana, Clonal Fidelity, RAPD, Primer
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How to cite this article:

Jotshana Manik Maske, Amol Dadarao Sable and Prashant Bhausaheb Kardile. 2018. Assessment of Clonal Fidelity and Genetic Analysis of Tissue Culture Raised Banana (Musa spp.) Plant.Int.J.Curr.Microbiol.App.Sci. 7(4): 2552-2558. doi: https://doi.org/10.20546/ijcmas.2018.704.292