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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2546-2551
DOI: https://doi.org/10.20546/ijcmas.2018.704.291


Detection and Identification of Tomato Leaf Curl Virus (ToLCV) using Molecular Technique - PCR Method
Jotshana Manik Maske1*, Amol Dadarao Sable1 and Prashant Bhausaheb Kardile2
1Department of Plant Biotechnology, SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.)-431001, India
2Department of Agricultural Botany, Dadasaheb Patil college of Agriculture, Dahegaon, Auranagabad (M.S.) – 423715, India
*Corresponding author
Abstract:

The cultivated tomato (Solanum lycopersicum L.) is economically one of the most important and widely grown plants of the Solanaceae family. Epidemics of Tomato leaf curl virus associated with upsurge of whiteflies (Bemasia tabacci) on tomato crop has been frequently reported which up to 100% yield losses (Shelat, et.al, 2014). In all twenty six samples were collected to see the presence of tomato leaf curl virus (ToLCV). Polymerase chain reaction (PCR) in the moist sensitive approach to detect a minute amount of viral nucleic acid it is the most method to amplified geminvirus as they replicate via-double-stranded, circular DNA from. In this study, geminivirus specific detergents primer was employed to detect ToLCV occurring in it vector whitefly Bemisia tabaci by PCR based approach. The presence of virus was observed through PCR method with specific primer CPAV1 forward and reveres on different annealing temperature range from 52ºC to 56ºC. The presence of virus was observed at 54ºC annealing temperature in sample no. 4, 11, 15, 22, 24, 25, 26) and confirm the presence of ToLCV.


Keywords: Tomato, ToLCV, PCR
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How to cite this article:

Jotshana Manik Maske, Amol Dadarao Sable and Prashant Bhausaheb Kardile. 2018. Detection and Identification of Tomato Leaf Curl Virus (ToLCV) using Molecular Technique - PCR Method.Int.J.Curr.Microbiol.App.Sci. 7(4): 2546-2551. doi: https://doi.org/10.20546/ijcmas.2018.704.291