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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2267-2271
DOI: https://doi.org/10.20546/ijcmas.2018.704.259


Quantitation of AFB1 by Spectrophotometric Method
B. Kisan, K. Shruthi Nagaral, A.S. Halepyati and I. Shankergoud
Department of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur, Karnataka, India
*Corresponding author
Abstract:

Aspergillus flavus produces potent aflatoxin and contaminates cereals, oil seeds and nuts, due to the climatic change the aflatoxin infestation is wide spread to various crops Viz: corn, soya, wheat, rice and cottonseeds, and nuts (peanut, almonds, Brazil nuts, hazelnuts, walnuts, cashew nuts, pecans, and pistachio nuts. Aflatoxin diet exposure has been observed through the consumption of foodstuffs, the contamination of maize, peanuts, and oilseeds. Aflatoxin causes deleterious health effects viz., human and animal health such as causing cancer, liver damage, diarrhoea, dizziness and fever are the common symptoms of aflatoxin contaminates food consumption. The aflatoxin contamination is observed pre and post-harvest, factors such as temperature, moisture, oxygen, and carbon dioxide, insect and rodent’s infestation, incidence of broken grains or nuts and the cleaning of the product will increase the incidence of toxigenic fungal load by transport, waiting time for drying, frying system and storage condition effect. Some countries established maximum concentration for aflatoxin in specific products ranging from (ppb to µg levels based on products, consumption pattern and country specific). The detection methods includes thin layer chromatography (TLC), high performance liquid chromatography (HPCL), mass spectroscopy, enzyme linked immuno sorbent assay (ELISA), and electrochemical immune sensor have been used for quantifying aflatoxins in foods and food commodities. These methods are costly, difficulty in performing assay and required technical knowhow. Hence, we have evaluated the inhibition of Acetyl choliesterase by aflatoxin (AFB1) for utility in assessing the presence of aflatoxine.AFB1 at132ng the inhibition obtained was 38.18%.Hence can be used for assessing aflatoxin contamination.


Keywords: Spectrophotometric, Aspergillus flavus
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How to cite this article:

Kisan, B., K. Shruthi Nagaral, A.S. Halepyati and Shankergoud, I. 2018. Quantitation of AFB1 by Spectrophotometric Method.Int.J.Curr.Microbiol.App.Sci. 7(4): 2267-2271. doi: https://doi.org/10.20546/ijcmas.2018.704.259