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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2224-2229
DOI: https://doi.org/10.20546/ijcmas.2018.704.253


Degradation of Discarded used Engine Oil by Pseudomonas aeruginosa DP-1 and its Optimization
Dipti R. Parikh1, Devayani R. Tipre1, Nisha S. Nayak1 and Shailesh R. Dave2*
1Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, India
2Xavier’s Research Foundation, Loyola Centre for Research and Development, St. Xavier College campus, Navrangpura, Ahmedabad – 380009, Gujarat, India
*Corresponding author
Abstract:

Oil degrading organisms were enriched in MSM medium supplemented with used engine oil. Organisms showed zone of clearance of 7-24 mm on TBA and 3-16 mm on used engine oil supplemented MSM plate. The % degradation of used engine oil was 14 to 37%. Isolate DP-1 showed highest activity, it was identified as Pseudomonas aeruginosa DP-1 by 16S r-RNA gene sequencing analysis. The organism gave 2, 6-dichlorophenol indophenol test positive. After optimization the used engine degradation was as high as 68% in 32 days of incubation. Selection and optimization resulted in 1.8 fold increase in used engine oil degradation potential of the organism.


Keywords: Pseudomonas aeruginosa; Used engine oil, Biodegradation, Optimization
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How to cite this article:

Dipti R. Parikh, Devayani R. Tipre, Nisha S. Nayak and Shailesh R. Dave. 2018. Degradation of Discarded used Engine Oil by Pseudomonas aeruginosa DP-1 and Its Optimization.Int.J.Curr.Microbiol.App.Sci. 7(4): 2224-2229. doi: https://doi.org/10.20546/ijcmas.2018.704.253