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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2219-2223
DOI: https://doi.org/10.20546/ijcmas.2018.704.252


A Comparative Study on the Detection of Intestinal Parasites by Using Different Methods from Stool Sample in a Tertiary Care Centre
Bhawna Kumari, Himadri Dutta and M. Kalyani
Department of Microbiology, Saveetha Medical Collegeand Hospital Saveetha University, Thandalam, Chennai-602105, Tamil Nadu, India
*Corresponding author
Abstract:

Parasitic infections, caused by intestinal helminths and protozoan parasites, are among the most prevalent infections in developing countries, while in developed countries, gastrointestinal infections are caused more commonly by protozoan parasites compared to helminths. A laboratory analysis of stool samples was done for detection of intestinal parasites in the Microbiology laboratory of a tertiary care hospital for a period of 6 months (February 2017 to July 2017). Stool samples were examined by different methods like direct smears (Saline and Lugol’s iodine) and concentration techniques. Formalin – ether sedimentation method and Zinc-sulphate floatation method and modified Ziehl Neelsen staining method. In our study a total of 231 stool samples were analysed and prevalence of intestinal parasitic infection was found to be 13.41% (31/231). There were five different parasites encountered. The most common parasite identified was Giardia lamblia (52%), followed by Entamoeba histolytica (19.35%), Hookworm (16.12%), Strongyloides stercoralis (6.45%) and Cryptosporidium parvum (6.45%).Use of concentration techniques definitely increased the detection rate when compared to conventional saline and iodine mount examination.


Keywords: Entamoeba histolytica, Giardia lamblia, Strongyloides stercoralis, Concentration techniques
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How to cite this article:

Bhawna Kumari, Himadri Dutta and Kalyani, M. 2018. A Comparative Study on the Detection of Intestinal Parasites by Using Different Methods from Stool Sample in a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 7(4): 2219-2223. doi: https://doi.org/10.20546/ijcmas.2018.704.252