Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2041-2057
DOI: https://doi.org/10.20546/ijcmas.2018.704.235


Prevalence of Carbapenem Resistant Klebsiella and Proteus Clinical Isolates: A Real Threat to the Egyptian Health Care System
Nourhan H. Fanaki, Hoda M. Omar, Amal M. Khalil and Eva A. Edward*
Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, 1 El Khartoum Sq., El Azareta, Alexandria, Egypt
*Corresponding author
Abstract:

The worldwide spread of carbapenem-resistant Enterobacteriaceae (CRE) has become a major challenge in healthcare settings. This study aimed to screen the susceptibility to imipenem and meropenem, among 49 Klebsiella and 7 Proteus vulgaris clinical isolates from Egypt. The combinations of carbapenems with ciprofloxacin, rifampicin, amikacin and colistin were tested against CRE. Attempts to cure plasmids mediating carbapenem resistance using menthol, as a natural curing agent, as well as to test its efficacy when combined with other antibiotics against CRE, were done. A comparison was done between phenotypic methods and polymerase chain reaction for the differentiation of carbapenemases. Among our isolates, 80.36 and 843.93% were resistant to imipenem and meropenem, respectively. The tested carabpenem/antibiotic combinations proved their efficacy against CRE. Menthol showed good curing rates (up to 100%) for carbapenem resistance plasmids. Also, in 71.43% of the studied cases, it improved the efficacy of the tested combinations. Polymerase chain reaction was more precise for the detection of carbapenemases. Among the tested isolates, blaNDM and blaOXA-48-like were the most prevalent carbapenemases. Our results demonstrated that carbapenem resistant Klebsiella and Proteus vulgaris clinical isolates have become a serious threat in Egypt. Combination therapy including menthol was the most appropriate for combating CRE.


Keywords: Klebsiella, Proteus vulgaris, Carbapenems, Menthol, Polymerase chain reaction, Phenotypic methods
Download this article as Download

How to cite this article:

Nourhan H. Fanaki, Hoda M. Omar, Amal M. Khalil and Eva A. Edward. 2018. Prevalence of Carbapenem Resistant Klebsiella and Proteus Clinical Isolates: A Real Threat to the Egyptian Health Care System.Int.J.Curr.Microbiol.App.Sci. 7(4): 2041-2057. doi: https://doi.org/10.20546/ijcmas.2018.704.235