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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2021-2025
DOI: https://doi.org/10.20546/ijcmas.2018.704.232


PCR as a Rapid Diagnostic Tool for Detection of Dermatophytes
Kalyani Putty2, J. Shiva Jyothi1, M. Sharanya2, M. Srikanth Reddy1, G. Sai Ram Sandeep1, M. Abhilash1, J. Venkatesh Yadav3, P. Purushotham1, I. Kesavulu Naidu2, A. Uma Chowdhary3, K. Sandhya Rani3, V. Pavani3, K. Vishwas3, B. Manoranjan Reddy3, Ch. Srinath4, P. Swapna4, A. Sreeja4, E. Kumar5, Y. Narasimha Reddy1 and K. Dhana Lakshmi1
1Department of Veterinary Microbiology
2Department of Veterinary Biotechnology
3Department of Veterinary Pathology
4Department of Veterinary Parasitology
5Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, P. V. N. R. Telangana Veterinary University, Rajendranagar, Hyderabad-500030, Telangana, India
*Corresponding author
Abstract:

Dermatophytes are a group of closely related keratinophilic fungi that can invade keratinized humans and animals tissues such as skin, hair and nails causing dermatophytosis. They are an important cause of superficial fungal infection. Conventional methods like potassium hydroxide (KOH) direct microscopy and fungal culture lacks the ability to make an early and specific diagnosis, as it takes about four weeks for the culture to grow on Sabourauds dextrose agar. So the present study was carried out by Polymerase chain reaction (PCR) using group specific primers targeting dermatophyte specific sequence of chitin synthase 1 (CHS1) gene with an estimated product size of 288bp. Hair follicles were collected from four different horses that were exhibiting Dermatophytosis clinical lesions on skin. DNA was extracted from hair follicles by Phenol chloroform method and the extracted DNA was subjected for PCR. Studies showed that all the 4 samples were positive for the dermatophytic infection as they had an expected product size at 288bp. Results indicate that PCR may be considered as rapid test for the diagnosis of dermatophytosis and can aid the clinician in initiating prompt and appropriate antifungal therapy. Early detection of such may further aid in the prevention of spreading and elimination of highly important zoonotic dermatophytes.


Keywords: Dermatophytes, PCR, Chitin synthase 1 gene
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How to cite this article:

Kalyani Putty, J. Shiva Jyothi, M. Sharanya, M. Srikanth Reddy, G. Sai Ram Sandeep, M. Abhilash, J. Venkatesh Yadav, P. Purushotham, I. Kesavulu Naidu, A. Uma Chowdhary, K. Sandhya Rani, V. Pavani, K. Vishwas, B. Manoranjan Reddy, Ch. Srinath, P. Swapna, A. Sreeja, E. Kumar, Y. Narasimha Reddy and Dhana Lakshmi, K. 2018. PCR as a Rapid Diagnostic Tool for Detection of Dermatophytes.Int.J.Curr.Microbiol.App.Sci. 7(4): 2021-2025. doi: https://doi.org/10.20546/ijcmas.2018.704.232