National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Dermatophytes are a group of closely related keratinophilic fungi that can invade keratinized humans and animals tissues such as skin, hair and nails causing dermatophytosis. They are an important cause of superficial fungal infection. Conventional methods like potassium hydroxide (KOH) direct microscopy and fungal culture lacks the ability to make an early and specific diagnosis, as it takes about four weeks for the culture to grow on Sabourauds dextrose agar. So the present study was carried out by Polymerase chain reaction (PCR) using group specific primers targeting dermatophyte specific sequence of chitin synthase 1 (CHS1) gene with an estimated product size of 288bp. Hair follicles were collected from four different horses that were exhibiting Dermatophytosis clinical lesions on skin. DNA was extracted from hair follicles by Phenol chloroform method and the extracted DNA was subjected for PCR. Studies showed that all the 4 samples were positive for the dermatophytic infection as they had an expected product size at 288bp. Results indicate that PCR may be considered as rapid test for the diagnosis of dermatophytosis and can aid the clinician in initiating prompt and appropriate antifungal therapy. Early detection of such may further aid in the prevention of spreading and elimination of highly important zoonotic dermatophytes.
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