National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A series of experiments were carried out with different combinations of various growth factors and hormones like Growth Hormone (GH), Insulin like Growth Factor-I (IGF-I), Epidermal Growth Factor (EGF), Thyroxin (T4) and Follicle Stimulating Hormone (FSH) in culture medium to support growth and development of goat preantral follicles (PFs) (diameter ranging from 150-400µ) in vitro for a period of eight days and the retrieved oocytes from cultured preantral follicles were kept for incubation in in vitro maturation media (IVM) for 27 hours. The development of the cultured PFs was assessed by the proportions of follicles exhibiting growth, increase in follicular diameter, antrum formation and extrusion of oocytes in vitro. During eight days culture period, the proportion of PFs exhibiting antrum formation (89.33±1.86) and extrusion of oocyte (15.55±2.09) were highest when the medium was supplemented with T4+FSH+GH+EGF (T3) having a significant difference with follicles cultured in other treatments. Oocytes retrieved from PFs grown in different culture media were kept in IVM media. Oocytes obtained from T3 treatment (T4+FSH+GH+EGF) had maximum percentage of M-II stage oocytes (38.6%) when compared to the other treatments. From the present study, it can be concluded that, in vitro development of goat PFs could be significantly improved through addition of GH, EGF, T4 and FSH in best combinations.
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