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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 1884-1889
DOI: https://doi.org/10.20546/ijcmas.2018.704.215


Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries
Kolipaka Rajesh1*, B. Swathi1, G. Aruna Kumari2 and M. Shanmugam3
1Department of Veterinary Physiology, College of Veterinary Science, Hyderabad - 500 030, India
2Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science, Korutla-505 326, India
3Department of Veterinary Physiology, DPR-ICAR Rajendranagar, ICAR- DPR, Hyderabad - 500 030, India
*Corresponding author
Abstract:

A study was taken up to know the effect of corpus luteum (CL) on the oocytes retrieval, quality in buffalo ovaries. Ovaries were collected from apparent healthy slaughtered buffalo during breeding season and categorized based on the presence (or) absence of CL. The cumulus oocyte complexes (COCs) were aspirated aseptically from the follicles of >6 mm diameter present on the surface of the ovary by using 18 G needle attached to 5 ml disposable syringe containing 2 ml of collection media. The oocytes were graded based on the presence of the cumulus cells complex around the oocytes. The mean A grade (3-5 cumulus cell layer) oocyte recovery rate per ovary was 1.35 from the ovaries with CL, while those without CL were 1.97, and the mean oocyte recovery rate per ovary was 1.78. In conclusion, an ovary without CL was found to be more suitable for harvesting higher number and superior quality of COCs.


Keywords: Corpus luteum, Oocytes retrieval, Cumulus oocyte complex, Oocytes per ovary
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How to cite this article:

Kolipaka Rajesh, B. Swathi, G. Aruna Kumari and Shanmugam, M. 2018. Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries.Int.J.Curr.Microbiol.App.Sci. 7(4): 1884-1889. doi: https://doi.org/10.20546/ijcmas.2018.704.215