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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 1835-1839
DOI: https://doi.org/10.20546/ijcmas.2018.704.208


Isolation and Microscopic Investigation of Entomopathogenic Nematodes (EPNs) Occurring in Barak Valley, Assam
Rupak Sharma1, 2*, Baby Singha1, Subhadeep Roy Choudhury2 and Gouriduttsharma3
1Department of Zoology, G.C College, Silchar, Assam, India
2Assam University, Department of Life Science and Bio-informatics, Silchar, Assam, India
3BilaspurUniversity, Chattishgarh, India
*Corresponding author
Abstract:

Entotomopathogenic nematodes (Steinernema sp.) isolated from the soil samples in the Barak Valley of Assam, India. The nematodes were isolated by baiting technique using greater wax moth (Galleria mellonella). Soil samples were collected during rabbi and monsoon season from the different locality of Barak Valley especially from crop fields, rubber plantation, pineapple garden, tea gardens and also from rhizospheric soil of bamboo bushes. Nematodes are isolated from rhizospheric soil of Bamboo and from crop field. Only on the basis of morphological characters isolates resembles with the Steinernema sp. Female having prominent vulva, anus, females juvenile bearing before emergence; males having prominent spicule and gubernaculum; juvenile with excretory pore anterior to nerve ring, tail is slightly curved and killed the insects larvae within 48 hours of infection. This is the first report for the occurrence of entomopathogenic nematodes from this region of Assam, India.


Keywords: Entomopathogenic nematodes, Crop Field, Steinernema sp., morphology, Barak Valley, Assam
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How to cite this article:

Rupak Sharma, Baby Singha, Subhadeep Roy Choudhury and Gouriduttsharma. 2018. Isolation and Microscopic Investigation of Entomopathogenic Nematodes (EPNs) Occurring in Barak Valley, Assam.Int.J.Curr.Microbiol.App.Sci. 7(4): 1835-1839. doi: https://doi.org/10.20546/ijcmas.2018.704.208