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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 1566-1578
DOI: https://doi.org/10.20546/ijcmas.2018.704.176


Prevalence, Virulence and Antibiotic Susceptibility of Listeria monocytogenes Recuperated from Slaughtered Goats and Pigs of Nagpur, Central India
G.R. Vaidya, S.P. Chaudhary, N.N. Zade, W.A. Khan, S.V. Shinde, A. Patil and D.G. Kalambhe*
Department of Veterinary Public Health Nagpur Veterinary College, MAFSU, Nagpur, India
*Corresponding author
Abstract:

Listeria monocytogenes is an emerging foodborne pathogen of public health concern. Animals may act an asymptomatic carrier and can contaminate foods of animal origin such as meats and dairy products. The objective of the study was to determine the prevalence, pathogenicity and antimicrobial profile of L. monocytogenes in goats and pigs intended for human consumption. A total of 350 samples comprising meat, milk, blood, and faeces of goats and pigs were analysed. Pathogen was isolated employing two step enrichment followed by plating on selective media. Isolates were characterized based on biochemical tests, haemolysis on blood agar, CAMP test, phosphatidyl inositol-specific phospholipase C assay and hlyA PCR. Antimicrobial sensitivity was assessed by disc diffusion method. Results of this study revealed7 (14%) and 10 (20%) prevalence of L. monocytogenes in goat and pigs respectively. All the isolates showed in-vitro virulence characteristics such as hemolysis on 5% sheep blood agar, positive CAMP test, PI-PLC activity and presence of hlyA gene. Antibiotic sensitivity assay showed that the isolates exhibited variable resistance pattern. Maximum sensitivity was observed for Penicillin-G and Cloxacilin whereas; resistance was noted towards Kanamycin, Rifampicin Cephotaxim., Oxytetracycline, Ceftriaxon, Ampicillin, Erythromycin, Norfloxacin and Gentamicin. This study highlights a potential risk of foodborne listeriosis for chevon and pork consumers of this region.


Keywords: Antimicrobial resistance, Goats, Listeria monocytogenes, Nagpur, Pigs
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How to cite this article:

Vaidya, G.R., S.P. Chaudhary, N.N. Zade, W.A. Khan, S.V. Shinde, A. Patil and Kalambhe, D.G. 2018. Prevalence, Virulence and Antibiotic Susceptibility of Listeria monocytogenes Recuperated from Slaughtered Goats and Pigs of Nagpur, Central India.Int.J.Curr.Microbiol.App.Sci. 7(4): 1566-1578. doi: https://doi.org/10.20546/ijcmas.2018.704.176