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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 1439-1447
DOI: https://doi.org/10.20546/ijcmas.2018.704.163


Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts
K.B. Joshi*, M.K. Mandavia and B.A. Golakiya
Department of Biochemistry and Biotechnology, Junagadh Agricultural University, Junagadh, India
*Corresponding author
Abstract:

The present study was designed to evaluate in vitro antimicrobial activity and antioxidant activity and anticancer activity of different five extracts of Euphorbia tirucalli Stem. Plant roots were extracted in different five solvents viz. Hexane, Chloroform, Methanol, Water and Methanol: Water (90:10) through Soxtherm according to polarity gradients. Aiming to investigate antimicrobial activities, agar well diffusion method was followed using five pathogenic bacteria and five fungi as test organisms. The plant stem extracts showed good antibacterial activities against Bacillus subtills and Psudomonas aruginosa which was compared with standard Chloramphenicol and Streptomycin, while 80% MeOH extract showed good antifungal activities (ZOI-13) against Fusarium oxysporum and (ZOI-18) against Sclerotium rolfsii. We assessed the antioxidant potential of all five extracts of Euphorbia tirucalli Stem using test involving inhibition of DPPH activities. The highest antioxidant activity of hexane and chloroform extracts were noticed at IC50 (Inhibition concentration at 50%) of 240 μg /ml and 350.480 μg /ml respectively, compared to those of ascorbic acid (7.5 μg /ml). The effects of plant stem extracts on four cancer cells (HeLa 229, A549, ACHN and KB 3-1) were studied. The highest IC50 (502.55 μg/ml to 118μg/ml.) values were found for the hexane and chloroform extracts respectively against A549 and ACHN Cell lines. This study validates the traditional use of the plant in management of Cancer. Current studies indicated that plant stem extracts possessed moderate antimicrobial activities and good antioxidant activity. So our findings revealed that the hexane and chloroform extracts of Euphorbia tirucalli Stem possess antioxidant and anticancer properties and could serve as free radical inhibitors or scavenger or, acting possibly as natural antioxidants, which may reduce the growth of Cancer cells and induce apoptosis in A549 and ACHN cell lines.


Keywords: Euphorbia tirucalli Stem, Antimicrobial activity, Antioxidant activity, DPPH, Anticancer activity, Trypan blue cell exclusion assay, MTT Assay
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How to cite this article:

Joshi, K.B., M.K. Mandavia and Golakiya, B.A. 2018. Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts.Int.J.Curr.Microbiol.App.Sci. 7(4): 1439-1447. doi: https://doi.org/10.20546/ijcmas.2018.704.163