In vitro Evaluation of the Curcumin against Fluconazole-Resistant Strains of Candida spp. and Cryptococcus neoformans
Daniel Domingues Freitas1, Cecília Rocha da Silva1, 2, João Batista de Andrade Neto1, Rosana de Sousa Campos1, 2, Letícia Serpa Sampaio1, Francisca Bruna Stefany Aires do Nascimento1, Maria Aparecida Alexandre Josino1, Lisandra Juvêncio da Silva1, Helaine Almeida Queiroz1, Fátima D. D. Barroso1, Jacó R. L. Mesquita3, Thalles Barbosa Granjeiro4, Said Gonçalves da Cruz Fonseca5, Bruno Coêlho Cavalcanti6, Manoel Odorico de Moraes6 and Hélio Vitoriano Nobre Júnior1*
1Department of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil
2Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil
3São José Hospital, Fortaleza, CE, Brazil
4Department of Biology, Science Center, Molecular Genetics Laboratory, Federal University of Ceara, CE, Brazil
5School of Pharmacy, Federal University of Ceara, Fortaleza, CE, Brazil
6Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, CE, Brazil
*Corresponding author
Abstract:
The isolates of Candida spp. and Cryptococcus neoformans were inhibited by curcumin, with different degrees of inhibition among species, with MICs ranging from 8-64 µg/mL. After exposure of the C. albicans strains to curcumin, it was observed a decrease in the number of viable cells and damage to cell membranes, the mitochondrial function of C. albicans cells appears to be affected after exposure to curcumin. Our data suggest that after exposure to curcumin, C. albicans cells showed total strand breaks in the DNA, where the nuclear DNA fragmentation was evident in the comet assay. Oxidative damage to DNA was verified by the alkaline comet assay in the presence of Fpg, where it was observed an increase in DNA migration of cells that were treated with curcumin. Based on the characteristics of cell death observed, it is raised the hypothesis that curcumin seems to exert its antifungal activity at specific sites near the DNA, resulting in cell death by apoptosis.
Keywords: Curcumin. Resistance. Fluconazole. Candida. Cryptococcus neoformans
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How to cite this article:
Daniel Domingues Freitas, Cecília Rocha da Silva, João Batista de Andrade Neto, Rosana de Sousa Campos, Letícia Serpa Sampaio, Francisca Bruna Stefany Aires do Nascimento, Maria Aparecida Alexandre Josino, Lisandra Juvêncio da Silva, Helaine Almeida Queiroz, Fátima D.D. Barroso, Jacó R.L. Mesquita, Thalles Barbosa Granjeiro, Said Gonçalves da Cruz Fonseca, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes and Hélio Vitoriano Nobre Júnior. 2018. In vitro Evaluation of the Curcumin against Fluconazole-Resistant Strains of Candida spp. and Cryptococcus neoformans.
Int.J.Curr.Microbiol.App.Sci. 7(4): 1191-1202. doi:
https://doi.org/10.20546/ijcmas.2018.704.132
Citations
National Academy of Agricultural Sciences (NAAS)
