National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out to develop an effective protocol for in vitro culture of Lilium longiflorum T. cv. Pavia. Three different types of explants like leaves, outer and inner bulb scales were exposed to two sterilants: sodium hypochlorite (NaClO) and hydrogen peroxide (H2O2) with their varying concentrations; NaClO (2%, 3%, 4% and 5%) and H2O2 (0.05%, 0.10%, 0.15% and 0.20%). Among all treatments, NaClO @ 3% was highly effective over different regime of H2O2 for minimizing contamination (18.66%), low necrosis (2.67%) and maximum survivability (79.13%). Highest bulblets induction (4.66) and maximum bulblets diameter (7.85 mm) was recorded in Murashige and Skoog (MS) medium supplemented with BAP (0.5 mg/l) and NAA (0.3 mg/l). The maximum length of shoots (38.33 mm) and fresh weight (239.51 mg) were recorded with BAP (1.0 mg/l) and NAA (0.1 mg/l). The maximum numbers of basal roots (5.83 /bulblets) were obtained with 0.3 mg/l NAA. The maximum length (49.00 mm) of basal roots was recorded with BAP (1.0 mg/l) and NAA (0.3 mg/l). This protocol is highly useful for mass multiplication of true-to-type, disease free planting material of lilium.
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