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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 1183-1190
DOI: https://doi.org/10.20546/ijcmas.2018.704.131


Standardization of an Effective Protocol for in vitro Culture of Lilium longiflorum Thunb cv. Pavia
N.S. Bhandari1 and C. Aswath2
1Division of Ornamental Crops, ICAR - Indian Agricultural Research Institute, New Delhi, India
2Division of Floriculture and Medicinal Crops, ICAR-Indian Institute of Horticultural Research, Bangalore, Karnataka, India
*Corresponding author
Abstract:

The present study was carried out to develop an effective protocol for in vitro culture of Lilium longiflorum T. cv. Pavia. Three different types of explants like leaves, outer and inner bulb scales were exposed to two sterilants: sodium hypochlorite (NaClO) and hydrogen peroxide (H2O2) with their varying concentrations; NaClO (2%, 3%, 4% and 5%) and H2O2 (0.05%, 0.10%, 0.15% and 0.20%). Among all treatments, NaClO @ 3% was highly effective over different regime of H2O2 for minimizing contamination (18.66%), low necrosis (2.67%) and maximum survivability (79.13%). Highest bulblets induction (4.66) and maximum bulblets diameter (7.85 mm) was recorded in Murashige and Skoog (MS) medium supplemented with BAP (0.5 mg/l) and NAA (0.3 mg/l). The maximum length of shoots (38.33 mm) and fresh weight (239.51 mg) were recorded with BAP (1.0 mg/l) and NAA (0.1 mg/l). The maximum numbers of basal roots (5.83 /bulblets) were obtained with 0.3 mg/l NAA. The maximum length (49.00 mm) of basal roots was recorded with BAP (1.0 mg/l) and NAA (0.3 mg/l). This protocol is highly useful for mass multiplication of true-to-type, disease free planting material of lilium.


Keywords: Lilium longiflorum, Sterilants, Explants, in vitro culture, Bulblets induction
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How to cite this article:

Bhandari, N.S. and Aswath, C. 2018. Standardization of an Effective Protocol for in vitro Culture of Lilium longiflorum Thunb cv. Pavia.Int.J.Curr.Microbiol.App.Sci. 7(4): 1183-1190. doi: https://doi.org/10.20546/ijcmas.2018.704.131