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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 978-989
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.110


Identification and Characterization of the Fibrinolytic Enzymes producing Bacterium Strain (Isolate GSM 55) from the Soil Waste
S.M. Gopinath* and K. Lingappa
Department of Microbiology, Gulbarga University, Gulbarga, Karnataka, India
*Corresponding author
Abstract:

The present work was conducted to characterize the  fibrinolytic enzyme produced by the strains of the isolates of  Streptomyces sp. obtained from the soil waste. The isolated organism was cultured in the sterile media at 37ºC for 24 hrs. The selected isolates were subjected to fibrinolytic activitiy. The fibrinolytic activity of isolates GSM 55 was higher and it was subjected for biochemical studies. Based on the study, isolate was designated as  Streptomyces aureus GSM 55. Further it was subjected to three step purification involving ammonium sulphate precipitation, Sephadex G-50 gel permeation chromatography and DEAE Sephadex A-50 anion exchange chromatography. After the purification processes, the molecular weight of fibrinolytic protease enzyme is  31kDa.


Keywords: Fibrinolytic activity, purification,   Sephadex G-50 gel permeation chromatography and DEAE Sephadex A-50 anion exchange chromatography
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How to cite this article:

Gopinath, S.M., and Lingappa, K. 2016. Identification and Characterization of the Fibrinolytic Enzymes producing Bacterium Strain (Isolate GSM 55) from the Soil Waste.Int.J.Curr.Microbiol.App.Sci. 5(7): 978-989. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.110