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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 894-906
DOI: https://doi.org/10.20546/ijcmas.2018.704.096


Isolation, Characterization and Identification of Probiotic Lactic Acid Bacteria (LAB) from Honey Bees
M. Mathialagan1*, Y.S. Johnson Thangaraj Edward1, P.M.M. David2, M. Senthilkumar3, M.R. Srinivasan1 and S. Mohankumar1
1Department of Agricultural Entomology
3Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
2Department of Agricultural Entomology, Agricultural College and Research Institute, Killikulam, Tamil Nadu, India
*Corresponding author
Abstract:

Laboratory experiments were conducted to explore the diversity of naturally-occurring probiotic lactic acid bacteria (LAB) associated with honey bees and to understand whether they can be used as probiotics to manage honey bee stressors. Honey stomach, honey, bee bread, bee pollen and royal jelly from different species of honey bees (Apis ceranaindica Fabricius, Apis melliefra Linnaeus, Apis florea Fabricius, Apis dorsata Fabricius, Tetragonula iridipennis Smith) were examined for the presence of LAB. The results indicated a rich diversity of LAB in the samples analysed, with 42 isolates belonging to six genera, viz., Enterococcus (23.8%), Micrococcus (18.8%), Streptococcus (13.8 %), Pediococcus (13.8 %), Lactobacillus (10.0%), Lactococcus (10.0 %) and Leuconostoc (10.0%). Their scope in disease management is discussed.


Keywords: Probiotic lactic acid bacteria (LAB), Diversity, Honey bee species
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How to cite this article:

Mathialagan, M., Y.S. Johnson Thangaraj Edward, P.M.M. David, M. Senthilkumar, M.R. Srinivasan and Mohankumar, S. 2018. Isolation, Characterization and Identification of Probiotic Lactic Acid Bacteria (LAB) from Honey Bees.Int.J.Curr.Microbiol.App.Sci. 7(4): 894-906. doi: https://doi.org/10.20546/ijcmas.2018.704.096