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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 969-976
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.109


Isolation and Screening of Fibrinolytic Enzymes producing Bacterium Strain from Soil Waste
S.M. Gopinath* and K. Lingappa
Department of Microbiology, Gulbarga University, Gulbarga, Karnataka, India
*Corresponding author
Abstract:

Fibrinolytic enzymes help in dissolving the fibrin clots and they have the potential use in the treatment of harmful cardiovascular diseases. High efficient fibrinolytic enzymes are produced from the fibrinolytic microbes under extreme environmental conditions. In the present work, microorganism strain that helps in the production of fibrinolytic enzymes were isolated and screened from the soil waste. Five isolates i.e., GSM-15, GSM -25, GSM -35, GSM -45 and GSM -55 were  subjected to macroscopic characteristics The isolated organism was cultured in the sterile media at 37ºC for 24 hrs. The selected isolates were subjected to caseinolytic, gelatinolytic and fibrinolytic activities was done. The fibrinolytic activity of isolates GSM 25 and GSM 55 was higher than that of caesinolytic and gelatinolytic activities. The selected isolates were tested with modified media compostition and at different pH values. Isolate GSM-55 showed good enzyme productivity (13.1 + 2.6) U/ml at pH 8.0. Also the biochemical study indicated the isolate GSM 55 is similar to that of Streptomyces sp. with fibrinolytic function.


Keywords: Fibrinolytic enzymes, isolates, fibrinolytic activity, caesinolytic and gelatinolytic activities
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How to cite this article:

Gopinath, S.M.,  and Lingappa, K. 2016. Isolation and Screening of Fibrinolytic Enzymes producing Bacterium Strain from Soil Waste.Int.J.Curr.Microbiol.App.Sci. 5(7): 969-976. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.109