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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 789-793
DOI: https://doi.org/10.20546/ijcmas.2018.704.088


Screening and Identification of SSR Markers for Genetic Diversity for Mungbean [Vigna radiata (L.) Wilczek]
M. Kanimoli Mathivathana1*, N. Jagadeeshselvam2, B. Madhumitha3, A. Karthikeyan4, M. Pandiyan5, G. Karthikeyan6, C. Vanniarajan1, M,Raveendran2, N. Senthil4* and M. Sudha2*
1Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Madurai, Tamil Nadu Agricultural university, Tamil Nadu, India
2Department of Plant Biotechnology, Center for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural university, Coimbatore, Tamil Nadu, India
3Department of pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India
4Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India
5Agricultural College and Research Institute, Tamil Nadu Agricultural University, Eachangkottai, Tamil Nadu, India
6Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
*Corresponding author
Abstract:

Mungbean (Vigna radiata) is an economically important pulse crop and is an excellent source of easily digestible proteins with low flatulence which complements the staple rice diet in Asia and particularly in India. Information about mungbean germplasm diversity and genetic relationships among accessions or cultivars is of fundamental importance for breeding and the management of germplasm. To study genetic diversity at molecular level different types of DNA markers can be used. Among the different DNA markers, simple sequence repeat (SSR) markers are highly variable in the genome, also reproducible and cost effective. A total of ten SSR primers that showed stable amplification was used to explore the diversity in six mungbean genotypes. Of these, two SSR primers VrSSR_6_ 746 and VrSSR_1_31 showed polymorphism in the six mungbean genotypes, while the remaining were monomorphic. Collectively, the results of present study can be helpful in planning the utilization patterns and management of mungbean genotypes.


Keywords: Genetic diversity, SSR, Polymorphism, Mungbean, Molecular markers
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How to cite this article:

Kanimoli Mathivathana, M., N. Jagadeeshselvam, B. Madhumitha, A. Karthikeyan, M. Pandiyan, G. Karthikeyan, C. Vanniarajan, M. Raveendran, N. Senthil and Sudha, M. 2018. Screening and Identification of SSR Markers for Genetic Diversity for Mungbean [Vigna radiata (L.) Wilczek].Int.J.Curr.Microbiol.App.Sci. 7(4): 789-793. doi: https://doi.org/10.20546/ijcmas.2018.704.088