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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 924-930
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.104


The Effect of Different Concentrations of the Run and Stuck Bacteria (Lactobacillus fermentum, Lactobacillus delbrueckii) in Trophozoite Growth Entamoeba histolytica in Vitro
Zainab Farooq Shafeek1* and Nada Sabah Ruzzuki2
1University of Al-Mustansiriyah, College of Pharmacy, Clinical Laboratory, Science Department, Iraq
2Baghdad University, College of sciences for Women, Biology Department, Iraq
*Corresponding author
Abstract:

This study describes the in vitro effect Lactobacillus fermentum, L.delbrueckii in Entamoeba histolytica was isolated from stools samples of patients with (amebic dysentery) and cultivated on Lock-egg medium to which sheep serum added. Than stud the effect of different concentrations (1.5102,1.51041.5108) of stuck (L.fermentum, L.delbruekii), and subject to examining after (24,28,72) hours of incubation, as well as to be observed the range of these concentrations' ability of the stuck and (L.fermentum, L.delbruekii) to reduce and resist the amebic.E. histolytica, additionally to note the changes in formation that parasites suffering from, during the period of treatment, Comparing with metronedizole (flagyl).The results showed the effectiveness of bacteria stuck in the inhibition proliferation of parasite growth a higher compared degree with the (L. fermentum, L.delbruekii) with the drug of metronidazole (Filagel) the treatment of effective concentrations reached to (1.5 102, 1.5 104, 1.5 108) of bacteria stuck (46.42, 88.65, 97.68)% respectively, after a 72h incubation. While the effectiveness of the treatment reached to (42.67, 31.57, 16.11) % respectively, after 48h incubation, while the (L. fermentum, L.delbruekii) showed less efficient compared to the treatment of metronidazole, It was noted the decomposition of the plasma membrane and the small size of amoeba from the natural state and discoloration phases with bad dye trypan blue.


Keywords: Lactobacillus fermentum, L.delbrueckii, Entamoeba histolytica.
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How to cite this article:

Zainab Farooq Shafeek and Nada Sabah Ruzzuki. 2016. The Effect of Different Concentrations of the Run and Stuck Bacteria (Lactobacillus fermentum, Lactobacillus delbrueckii) in Trophozoite Growth Entamoeba histolytica in Vitro.Int.J.Curr.Microbiol.App.Sci. 5(7): 924-930. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.104