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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 683-694
DOI: https://doi.org/10.20546/ijcmas.2018.704.077


Green Synthesis of Copper Nano-Particles Using Asparagus adscendens Roxb. Root and Leaf Extract and Their Antimicrobial Activities
Sapna Thakur1*, Sushma Sharma2, Shweta Thakur3 and Radheshyam Rai4
1Department of Biotechnology
2Department of Agriculture, Akal College of Agriculture, Eternal University, Himachal Pradesh, India
3Department of Physics, University of Aveiro, 3810-193 Aveiro, Portugal
4Department of Physics, Eternal University, Himachal Pradesh, India
*Corresponding author
Abstract:

Bio-nanotechnology has provides extensive research with emergence in biotechnology and nanotechnology which synthesize nanoparticles using bio-organisms like plant parts, bacteria yeasts, algae and fungi and that is more compatible with the green chemistry principles. Copper nanoparticles were biologically synthesized by using the root extract and leaf extract of Asparagus adscendens Roxb. as a reducing agent. By treatment of aqueous solutions of CuSO4•5H2O with root and leaf extract stable copper nanoparticles were formed. UV-Visible study revealed quantitative formation of copper nanoparticles or characteristic absorption peak of Cu nano-particles in both cases at the range of 500-700nm. These biosynthesized Cu nanoparticles were characterized with the help of Fourier transform infrared spectroscopy (FTIR) and High Resolution Transmission Electron Microscopy (HRTEM). The involvement of proteins and possible reducing agent was confirmed by FTIR analysis. HRTEM confirms the formation and the crystalline nature of copper nanomaterial. Thus, this method can be used as a rapid and environmental friendly, non-toxic and safe biological reagents approach for copper nanoparticles synthesis. The copper nanoparticles were isolated from these herbal roots and leaves tested for antimicrobial activity. The test cultures included in this study were Escherichia coli, Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, and Staphylococcus aureus. This study scientifically revealed significant zone of inhibition against such pathogenic bacteria’s which has many pharmaceutical applications for the control of deadly pathogens.


Keywords: Green synthesis, Copper nanoparticals, Asparagus adscendens Roxb.
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How to cite this article:

Sapna Thakur, Sushma Sharma, Shweta Thakur and Radheshyam Rai. 2018. Green Synthesis of Copper Nano-Particles Using Asparagus adscendens Roxb. Root and Leaf Extract and Their Antimicrobial Activities.Int.J.Curr.Microbiol.App.Sci. 7(4): 683-694. doi: https://doi.org/10.20546/ijcmas.2018.704.077