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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 641-647
DOI: https://doi.org/10.20546/ijcmas.2018.704.072


SSR Marker Based DNA Fingerprinting for Cowpea Varieties of Tamil Nadu [Vigna unguiculata (L.) Walp.]
S. Ragul, N. Manivannan, A. Mahalingam*, V. Babu Rajendra Prasad and S.Lakshmi Narayanan
National Pulses Research Centre, Tamil Nadu Agricultural University Vamban, Pudukkottai – 622 303, Tamil Nadu, India
*Corresponding author
Abstract:

Morphological traits viz., flower colour, leaf shape, seed colour and seed shape are available to distinguish the new cowpea variety VBN 3 from the ruling varieties Vamban 1, and CO(CP) 7. However, all these varieties cannot be distinguished with a single character alone. A total of 25 SSR primers were used to differentiate VBN 3 from Vamban 1 and CO(CP) 7. Among these six primers viz., CEDG156, CP09781, CEDG171, CEDG127, CEDG008 and CEDG 305 had polymorphism among varieties. Primers viz., CEDG156, CP09781, CEDG171 and CEDG008 had polymorphism between Vamban 1 and VBN 3. Likewise, primers CEDG 156, CP09781, CEDG127 and CEDG305 had polymorphism between VBN 3 and CO(CP) 7. One primer, CP09781 was able to differentiate all three varieties. Hence these primers may be useful to differentiate these varieties at DNA level. These markers will be a potent tool in seed certification.


Keywords: Cowpea, SSR Marker, DNA fingerprinting
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How to cite this article:

Ragul, S., N. Manivannan, A. Mahalingam, V. Babu Rajendra Prasad and Lakshmi Narayanan, S. 2018. SSR Marker Based DNA Fingerprinting for Cowpea Varieties of Tamil Nadu [Vigna unguiculata (L.) Walp.].Int.J.Curr.Microbiol.App.Sci. 7(4): 641-647. doi: https://doi.org/10.20546/ijcmas.2018.704.072