National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Morphological traits viz., flower colour, leaf shape, seed colour and seed shape are available to distinguish the new cowpea variety VBN 3 from the ruling varieties Vamban 1, and CO(CP) 7. However, all these varieties cannot be distinguished with a single character alone. A total of 25 SSR primers were used to differentiate VBN 3 from Vamban 1 and CO(CP) 7. Among these six primers viz., CEDG156, CP09781, CEDG171, CEDG127, CEDG008 and CEDG 305 had polymorphism among varieties. Primers viz., CEDG156, CP09781, CEDG171 and CEDG008 had polymorphism between Vamban 1 and VBN 3. Likewise, primers CEDG 156, CP09781, CEDG127 and CEDG305 had polymorphism between VBN 3 and CO(CP) 7. One primer, CP09781 was able to differentiate all three varieties. Hence these primers may be useful to differentiate these varieties at DNA level. These markers will be a potent tool in seed certification.
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