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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 627-635
DOI: https://doi.org/10.20546/ijcmas.2018.704.070


Mycelia Growth and Spore Yield of Trichoderma harzianum in Batch and Fed-Batch Cultures: Influence of pH and Temperature
Abiodun A. Onilude and Damilola O. Seyi-Amole*
Microbial Physiology and Biochemistry Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Nigeria
*Corresponding author
Abstract:

Trichoderma harzianum is a biocontrol agent that has moderate effect on soil balance and no harmful effect on other beneficial organisms in the soil. In this study we examined the effect of pH and temperature on the mycelia growth and spore yield of T. harzianum in batch and fed-batch culture. The pH and temperature had significant effect on the growth and sporulation of T. harzianum and this was dependent on the cultivation technique. It was observed that the optimum pH for maximum mycelial growth and spore yield produced by T. harzianum in batch and fed-batch cultures was pH 4 while the optimum temperature for mycelial growth in batch and fed-batch culture was 30 oC and the maximum spore yield was produced at optimum temperature of 25 oC and 45 oC in batch and fed- batch culture respectively. The results showed that fed-culture produced more spore yield than batch culture while high mycelial growth was obtained in batch culture. This work has revealed the important role that environmental conditions plays in the mycelia growth and spore yield of T. harzianum, a biocontrol agent.


Keywords: Trichoderma harzianum, Batch culture, Fed-batch culture, pH, Temperature
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How to cite this article:

Abiodun A. Onilude and Damilola O. Seyi-Amole. 2018. Mycelia Growth and Spore Yield of Trichoderma harzianum in Batch and Fed-Batch Cultures: Influence of pH and Temperature.Int.J.Curr.Microbiol.App.Sci. 7(4): 627-635. doi: https://doi.org/10.20546/ijcmas.2018.704.070