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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 548-555
DOI: https://doi.org/10.20546/ijcmas.2018.704.064


Genetic Diversity Analysis of Rice Landraces of NW Himalayas Using RAPD and ISSR Markers
Ritu Sood, Sanchit Thakur, Neelam Bhardwaj* and R.P. Kaushik
College of Agriculture, CSK Himachal Pradesh Agricultural University, Palampur-176062, India
*Corresponding author
Abstract:

Forty seven landraces of rice collected from different agro-climatic zones of Himachal Pradesh along with three check varieties were used in the present investigation for the diversity studies using molecular markers to know the their genetic relationship. Higher level of polymorphism was detected by both RAPD and ISSR analysis but the ISSR polymorphism percentage was higher than that of RAPD. Using fifteen RAPD and eleven ISSR primers, 81.2 and 86.4 percent of DNA polymorphism could be detected among these genotypes. The average Jaccard’s similarity coefficient based on RAPD, ISSR and combined RAPD and ISSR analysis was 0.49, 0.62 and 0.57, respectively. Combined analysis of RAPD and ISSR showed that out of 50 landraces, 44 are grouped in Cluster I. Grouping of majority of the genotypes into one cluster suggested affinity among the genotypes indicating their origin in same geographical area. RAPD showed the landraces ‘IC 3131155’ and ‘Sukara’ as most divergent ones, while the land races ‘Local Variety’ and ‘Lalzhini’ are most diverse as per the ISSR result. The present study revealed the existence of sufficient amount of genetic variability among the landraces, which could be exploited further in the breeding programme.


Keywords: RAPD, ISSR, Genetic diversity, Rice landraces
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How to cite this article:

Ritu Sood, Sanchit Thakur, Neelam Bhardwaj and Kaushik, R.P. 2018. Genetic Diversity Analysis of Rice Landraces of NW Himalayas Using RAPD and ISSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(4): 548-555. doi: https://doi.org/10.20546/ijcmas.2018.704.064