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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 961-968
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.108


Molecular Detection of Salmonella Isolated from Poultry Farms in Abia State Southeast Nigeria
Nwiyi Paul*, F. Chah Kennedy and S.V.O. Shoyinka
 Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria
*Corresponding author
Abstract:

The study was conducted in six local government areas of Abia State, and this includes Aba north and south, Umuahia south and Ikwuano, Ohafia and Bendel local government areas and three senatorial zones that make up the State, namely, Abia south, Abia central and Abia north respectively. The aim of the study was to detect the presence of Salmonella isolated from poultry farms using molecular method. A total of 1420 samples were collected comprising 800 eggs, 420 clocal swabs and 200 poultry litter. The samples were processed in the veterinary microbiology laboratory. Universal primer set specific for genus Salmonella 16SrDNA 341F (5-CCTACGGGAGGCAGCAG-3 and 907R5-CCGTCATTT CCTTTRAGTTT-3) was used. The result show that a total of 28 Salmonella were isolated with egg, cloacal swab and litter presenting 18, 7 and 3 Salmonella distribution respectively and isolation rate of 1.97%. There were no significant association (p> 0.05) between rate of isolation and senatorial zone. The molecular study using agarose gel of amplification products shows that there was detection of Salmonella species with bands at 200bp.


Keywords: Salmonella,Poultry Farms,Avian Salmonellosis
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How to cite this article:

Nwiyi Paul, F. Chah Kennedy and S.V.O. Shoyinka. 2016.  Molecular Detection of Salmonella Isolated from Poultry Farms in Abia State Southeast NigeriaInt.J.Curr.Microbiol.App.Sci. 5(7): 961-968. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.108