National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was conducted in six local government areas of Abia State, and this includes Aba north and south, Umuahia south and Ikwuano, Ohafia and Bendel local government areas and three senatorial zones that make up the State, namely, Abia south, Abia central and Abia north respectively. The aim of the study was to detect the presence of Salmonella isolated from poultry farms using molecular method. A total of 1420 samples were collected comprising 800 eggs, 420 clocal swabs and 200 poultry litter. The samples were processed in the veterinary microbiology laboratory. Universal primer set specific for genus Salmonella 16SrDNA 341F (5-CCTACGGGAGGCAGCAG-3 and 907R5-CCGTCATTT CCTTTRAGTTT-3) was used. The result show that a total of 28 Salmonella were isolated with egg, cloacal swab and litter presenting 18, 7 and 3 Salmonella distribution respectively and isolation rate of 1.97%. There were no significant association (p> 0.05) between rate of isolation and senatorial zone. The molecular study using agarose gel of amplification products shows that there was detection of Salmonella species with bands at 200bp.
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