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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 333-341
DOI: https://doi.org/10.20546/ijcmas.2018.704.038


Identification and Utilization of Polymorphic SSR Markers for Genetic Diversity Studies in Oil Palm
H.P. Bhagya1*, B. Kalyana Babu1, Mahanthesha B.N. Naika2, R.K. Mathur1, P.M. Gangadharappa3, D. Satisha2 and R.B. Naik4
1ICAR-Indian Institute of Oil palm Research, Pedavegi, West Godavari District, Andhra Pradesh, India
2Kittur Rani Channamma College of Horticulture, Arabhavi-591218 UHS, Bagalkot, Karnataka, India
3College of Horticulture, Munirabad, UHS, Bagalkot, Karnataka, India
4RHREC, Kumbapur, Dharwad, Karnataka, India
*Corresponding author
Abstract:

Oil palm (Elaeis guineensis Jacq.) having chromosome number 2n=32 and belongs to the family Arecaceae and it is a rich source of perennial vegetable-oil in India. To sustain the edible oil requirement in the country, oil palm is one of the best options due to its high oil-yield (4-6 t/ha) potential compared to other annual oil-yielding crops. Polymorphic simple sequence repeat (SSR) markers play an important role in genetic diversity and mapping studies in a crop like oil palm. In the present study, total eight genotypes were screened using 110 SSR markers. With of these, 42 were found to be polymorphic and 68 were monomorphic and the number of alleles ranged from two to six. The highest Polymorphism Information Content (PIC) value was observed with the primer, mEgCIR0779 (0.76), while the lowest with mEgCIR3288 (0.11), at an average value of 0.38. Genetic diversity ranged from 0.12 (mEgCIR3288) to 0.79 (mEgCIR0779), with an average value of 0.45. Based on PIC and other genetic parameters, four highly-polymorphic markers, viz., mEgCIR0779, mEgCIR0782, mEgCIR2347 and mEgCIR2595 were identified. The identified polymorphic SSR loci can be effectively used in mapping and genetic diversity studies of oil palm crop improvement programme. Totally 42 polymorphic SSRs identified and grouped the eight genotypes into two major clusters and the clustering pattern observed was that based on geographical origin.


Keywords: Cluster, Elaeis guineensis, Genotypes, Molecular, PIC
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How to cite this article:

Bhagya, H.P., B. Kalyana Babu, Mahanthesha B.N. Naika, R.K. Mathur, P.M. Gangadharappa, D. Satisha and Naik, R.B. 2018. Identification and Utilization of Polymorphic SSR Markers for Genetic Diversity Studies in Oil Palm.Int.J.Curr.Microbiol.App.Sci. 7(4): 333-341. doi: https://doi.org/10.20546/ijcmas.2018.704.038