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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 317-324
DOI: https://doi.org/10.20546/ijcmas.2018.704.036


Liquid Formulation Using Different Oils and Shelf Life Study of Effective Bacterial Bio-Agents
S.M. Jayasudha*, K.C. Kirankumar, R.K. Mesta and Ramesh Ippikoppa
Department of Plant Pathology, University of Horticultural sciences, Bagalkot-587103, Karnataka, India
*Corresponding author
Abstract:

Liquid formulation of Bacillus subtilis-KK-9A, Brevibacillus borstelensis-BK-6, Brevibacillus sp-PM-2A, Lysinibacillus xylanilyticus-VK-6B and consortium were developed using distilled water, ground nut oil, nutrient broth, pongamia oil and sunflower oil to enhance the shelf life and efficacy of the biocontrol agent besides easing the delivery of bio-inoculants through micro-irrigation techniques. The highest population level of Bacillus subtilis-KK-9A, Brevibacillus borstelensis-BK-6, Brevibacillus sp-PM-2A and Lysinibacillus xylanilyticus-VK-6B were observed in pongamia oil amended with glycerol even after 3 months of storage. Pongamia oil based formulation was found best formulation followed by ground nut oil, sunflower oil, distilled water, nutrient broth based formulation which retains the highest per cent survival of Bacillus subtilis (45.05), Brevibacillus borstelensis (32.93), Brevibacillus sp (48.80), Lysinibacillus xylanilyticus (60.80), and consortium 31.12 per cent even after 90 days.


Keywords: Bacterial bio-agents, Formulation, Shelf life
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How to cite this article:

Jayasudha, S.M., K.C. Kirankumar, R.K. Mesta and Ramesh Ippikoppa. 2018. Liquid Formulation Using Different Oils and Shelf Life Study of Effective Bacterial Bio-Agents.Int.J.Curr.Microbiol.App.Sci. 7(4): 317-324. doi: https://doi.org/10.20546/ijcmas.2018.704.036