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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 931-943
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.105


Phenotypic and Genotypic Diversity of Nosocomial Multi-Drug Resistant Klebsiella pneumoniae Isolated from Cancer Patients in Cairo, Egypt
Mahmoud M. Tawfick1,2*, Samira M. Hamed2, Hadeel M. Darwich2 and Hadir A. El-Mahallawy3
1Microbiology and Immunology Department, Faculty of Pharmacy,Al-Azhar University, Cairo, Egypt
2Microbiology and Immunology Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Cairo, Egypt
3Clinical Pathology, Medical Oncology Department, National Cancer Institute,Cairo University, Egypt
*Corresponding author
Abstract:

Nosocomial Klebsiella pneumoniae infections are particularly a problem among cancer and immunocompromised patients worldwide. K. pneumoniae strains are widespread in nature thus typing is required to discriminate them in the epidemiological investigations. In this study, the diversity of 43 nosocomial multi-drug resistant (MDR) K. pneumoniae isolates recovered from different clinical specimens collected from cancer patients at National Cancer Institute, Cairo, Egypt, were phenotypically and genotypically analysed. These isolates were identified using conventional microbiological methods and the API 20E system. Investigation of the antimicrobial susceptibility patterns against 16 diverse antimicrobial agents revealed that all isolates are MDR. The phenotyping was performed using the API 20E-based biotyping and antibiogram typing which showed three different biotypes and 25 antibiogram types among the isolates. The genotyping using random amplified polymorphic DNA (RAPD) analyses revealed 39 different RAPD-based fingerprints and/or 43 different patterns among the isolates. In conclusion, K. pneumoniae infections in this institution have been caused by diverse MDR K. pneumoniae genotypes and/or phenotypes clone groups with isolates in the same phenotype group possess different genotypes. Biotyping and antibiogram typing of K. pneumoniae isolates have been shown to be well for preliminary screening of strain relatedness. The use of RAPD-PCR-based analyses is recommended, which has high discriminatory power providing definite information to evaluate the epidemic status of the nosocomial infections caused by MDR K. pneumoniae.


Keywords: Klebsiella pneumoniae, RAPD-PCR,multi-drug resistant, typing, genetic diversity.
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How to cite this article:

Mahmoud M. Tawfick, Samira M. Hamed, Hadeel M. Darwich and Hadir A. El-Mahallawy. 2016. Phenotypic and Genotypic Diversity of Nosocomial Multi-Drug Resistant Klebsiella pneumoniae Isolated from Cancer Patients in Cairo, Egypt.Int.J.Curr.Microbiol.App.Sci. 5(7): 931-943. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.105