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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Green gram bacterial leaf spot pathogen X. axonopodis pv. vignaeradiatae was isolated from diseased samples collected from different locations of Udaipur district. The pathogenicity was proved on one month old green gram plants of cv. SML-668 and isolate Xav-02 (Bhatewar) was found more virulent with 4 days incubation period and require 12 days for disease development. Initially dark brown water soaked spots were found on cotyledons and then appeared on leaves and stem of seedlings. On leaves, irregular dark brown raised necrotic spots were developed with shot holes. Further, elongated dark brown spots with cracks were detected on stems. Whereas, dark brown necrotic spots were developed on pods and infected pods had small, shrivelled, deformed and discolored seeds. Thus diseased plants had poor standing without mortality. To find out most convenient and efficient method for disease production five different artificial inoculation methods were used such as (1) Spraying of bacterial suspension once, (2) Spraying of bacterial suspension twice, (3) Spraying of bacterial suspension thrice, (4) Rubbing bacterial suspension with carborundum powder and (5) Spraying of bacterial suspension after multi needle pricking. The spraying of bacterial suspension thrice method gave quicker symptoms expression in potted plants as well as field conditions, respectively.