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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 152-166
DOI: https://doi.org/10.20546/ijcmas.2018.704.017


Pathogenicity Test and Convenient Inoculation Method of Xanthomonas axonopodis pv. vignaeradiatae Caused Leaf Spot of Green gram
Jitendra Kumar* and Anila Doshi
Department of Plant Pathology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur-313001, India
*Corresponding author
Abstract:

Green gram bacterial leaf spot pathogen X. axonopodis pv. vignaeradiatae was isolated from diseased samples collected from different locations of Udaipur district. The pathogenicity was proved on one month old green gram plants of cv. SML-668 and isolate Xav-02 (Bhatewar) was found more virulent with 4 days incubation period and require 12 days for disease development. Initially dark brown water soaked spots were found on cotyledons and then appeared on leaves and stem of seedlings. On leaves, irregular dark brown raised necrotic spots were developed with shot holes. Further, elongated dark brown spots with cracks were detected on stems. Whereas, dark brown necrotic spots were developed on pods and infected pods had small, shrivelled, deformed and discolored seeds. Thus diseased plants had poor standing without mortality. To find out most convenient and efficient method for disease production five different artificial inoculation methods were used such as (1) Spraying of bacterial suspension once, (2) Spraying of bacterial suspension twice, (3) Spraying of bacterial suspension thrice, (4) Rubbing bacterial suspension with carborundum powder and (5) Spraying of bacterial suspension after multi needle pricking. The spraying of bacterial suspension thrice method gave quicker symptoms expression in potted plants as well as field conditions, respectively.


Keywords: Bacterial suspension, Green gram, Artificial inoculation, Pathogenicity, Spray
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How to cite this article:

Jitendra Kumar and Anila Doshi. 2018. Pathogenicity Test and Convenient Inoculation Method of Xanthomonas axonopodis pv. vignaeradiatae Caused Leaf Spot of Green gram.Int.J.Curr.Microbiol.App.Sci. 7(4): 152-166. doi: https://doi.org/10.20546/ijcmas.2018.704.017