National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Anthers from four genotype of Indian mustard, namely Local Yella (CAULC-1), JD-6, Kranti and NDRE-22) were cultured in vitro to observe their androgenic responses. Different concentrations and combinations of growth regulators were supplemented in B5 and MS medium. The range of callus induction was between 16.11 and 66.39%. The maximum rate of callus induction in terms of percentage (%) was observed in MS + 3 ml/l 2, 4-D + 1ml/l BAP (66.39). Among the Genotypes CAULC-1 (Local Yella) showed the best performance for days to callus initiation and per cent of callus induction while NDRE-22 was the poorest. Maximum per cent of shoot initiation at 30 and 45 DAI (55.55% and 59.44% respectively) was observed in MS + 4 ml/l BAP + 1 ml/l NAA. Local Yella (CAULC-1) recorded the best for shoot regeneration followed by Kranti. The media composition MS + 1 ml/l NAA + 0.5 ml/l BAP showed the minimum days (12.97) and highest per cent of root initiation (45.83%) and also maximum length of regenerated root (3.42 cm) respectively. Local Yella was found to be the best genotype which took minimum time (12.44 days). Regeneration of haploid plants was confirmed by cytological examinations of the root tips of the plantlets from the callus of anther culture. The present study can be concluded that Local Yella (CAULC-1) showed better androgenic response than other genotypes under study.
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