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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 8-19
DOI: https://doi.org/10.20546/ijcmas.2018.704.002


In vitro Haploid Plantlet Regeneration through Anther Culture in Locally Adapted Cultivar of Indian Mustard (Brassica juncea L. Czern and Coss)
N. Reetisana1, Th. Renuka Devi1*, H. Nanita Devi2, J.M. Laishram1 and Artibashisha Hijam Pyngrope1
1College of Agriculture, Central Agricultural University, Iroisemba, Imphal, Manipur (795 004), India
2AICRP (Soybean), Directorate of Research, C. A. U., Lamphelpat, Imphal, Manipur (795 004), India
*Corresponding author
Abstract:

Anthers from four genotype of Indian mustard, namely Local Yella (CAULC-1), JD-6, Kranti and NDRE-22) were cultured in vitro to observe their androgenic responses. Different concentrations and combinations of growth regulators were supplemented in B5 and MS medium. The range of callus induction was between 16.11 and 66.39%. The maximum rate of callus induction in terms of percentage (%) was observed in MS + 3 ml/l 2, 4-D + 1ml/l BAP (66.39). Among the Genotypes CAULC-1 (Local Yella) showed the best performance for days to callus initiation and per cent of callus induction while NDRE-22 was the poorest. Maximum per cent of shoot initiation at 30 and 45 DAI (55.55% and 59.44% respectively) was observed in MS + 4 ml/l BAP + 1 ml/l NAA. Local Yella (CAULC-1) recorded the best for shoot regeneration followed by Kranti. The media composition MS + 1 ml/l NAA + 0.5 ml/l BAP showed the minimum days (12.97) and highest per cent of root initiation (45.83%) and also maximum length of regenerated root (3.42 cm) respectively. Local Yella was found to be the best genotype which took minimum time (12.44 days). Regeneration of haploid plants was confirmed by cytological examinations of the root tips of the plantlets from the callus of anther culture. The present study can be concluded that Local Yella (CAULC-1) showed better androgenic response than other genotypes under study.


Keywords: Anther culture, Angrogenic, Brassica juncea, in-vitro regeneration
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How to cite this article:

Reetisana, N., Th. Renuka Devi, H. Nanita Devi, J.M. Laishram and Artibashisha Hijam Pyngrope. 2018. In vitro Haploid Plantlet Regeneration through Anther Culture in Locally Adapted Cultivar of Indian Mustard (Brassica juncea L. Czern and Coss).Int.J.Curr.Microbiol.App.Sci. 7(4): 8-19. doi: https://doi.org/10.20546/ijcmas.2018.704.002