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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3356-3366
DOI: http://dx.doi.org/10.20546/ijcmas.2017.606.394


Cinicomycological study of otomycosis with antifungal drug susceptibility testing of Candida isolates using disk diffusion method in Kota region, Rajasthan, India
Rawat Sarita*, Saxena Naveen, Chand–E-Anita, Garg Namita, Verma Vikas and Sharma Khushboo
Department of Microbiology, GMC Kota, Rajasthan, India
*Corresponding author
Abstract:

Otomycosis or fungal otitis externa is a superficial, sub-acute or chronic infection of the external auditory canal. Its prevalence has been quoted to range from 9% to 27%. The aim of the study is to find the prevalence of otomycosis along with its clinicomycological profile and to detect antifungal drug susceptibility of Candida isolates by disk diffusion method. A total of 100 samples were collected using swabs from ENT OPD based on symptoms and otoscopic finding suggestive of otomycosis and were processed in mycology lab. Otomycosis was diagnosed in 94 % of the cases with highest prevalence in 11-30 years of age group. Male to female ratio was 1.12:1. Aspergillus niger (58%) was the predominant isolate followed by Aspergillus flavus (23%), Candida spp (12%), Aspergillus fumigatus (4%), Penicillium (1%), Geotrichum (1%) and scopulariopsis (1%). Among Candida species, Candida albicans (50%), C. tropicalis (25%), Candida glabrata (16.66%) and Candida kefyr (8.33%) were isolated. Antifungal drug susceptibility testing results shows 100% sensitivity to Amphotericin B for all candida isolates. Resistance against fluconazole was present in 16 % of C.albicans isolates, 25% of C. tropicalis isolates. Nystatin was resistance among 16% of C.albicans & 25% of C. tropicalis, whereas clotrimazole resistance was present in 23% of C.albicans, 25% of C. tropicalis & 50% of C.glabrata isolates. Ketoconazole was resistant among 16% of C. albicans. Our study showed a high prevalence of otomycosis in the Kota region, thus proper diagnosis and treatment by aseptic techniques for this disease is required.


Keywords: Otomycosis, Aspergillus niger, Antifungal drug susceptibility

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How to cite this article:

Rawat Sarita, Saxena Naveen, Chand–E-Anita, Garg Namita, Verma Vikas and Sharma Khushboo. 2017. Cinicomycological study of otomycosis with antifungal drug susceptibility testing of Candida isolates using disk diffusion method in Kota region, Rajasthan.Int.J.Curr.Microbiol.App.Sci. 6(6): 3356-3366. doi: http://dx.doi.org/10.20546/ijcmas.2017.606.394
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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