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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3681-3690
DOI: https://doi.org/10.20546/ijcmas.2018.703.426


Genetic Diversity Study of Clarias magur using Simple Sequence Repeats
Mukesh Kumar1, Aditya Pratap Acharya1, Sujit Kumar2, P. Gireesh Babu1*,Pavan Kumar1 and Aparna Chaudhari1
1Fish Genetics and Biotechnology Division, ICAR – Central Institute of Fisheries Education, Panchmarg, Off Yari Road, Mumbai-400061, India
2Aquaculture Departments, Kamdhenu University, Gandhinagar, Gujarat-382010, India
*Corresponding author
Abstract:

Microsatellites or simple sequence repeats (SSR) are widely used in population genetic analysis because of their higher polymorphism, abundance, and co-dominance. The present work was taken to develop a specific primer for novel microsatellite loci and to validate them. Total 25 primer pairs were designed of which six primer pairs were found to produce specific band of the desired size. These six primers were validated using samples collected from Andhra Pradesh (Godavari River), Assam (Brahmaputra River), and West Bengal (Hooghly River). Out of six, five loci were found polymorphic with a 2-4 number of alleles per locus. Among the five loci, Cb21GACA locus showed maximum alleles (3-4) with highest PIC value (0.664) while locus CbACT6-GT25 showed less PIC value (0.164). The highest effective number of alleles was 2.721 in Cb21GACA and the lowest effective number was 1.241 of CbACT6-GT25. The mean effective number of alleles for overall populations was 1.847. The observed and expected heterozygosis was ranged from 0.05 to 0.80 and 0.095 to 0.66, respectively. The FST (0.164) and a number of migrants values (0.718) indicate a moderate level of population sub-structuring.


Keywords: Microsatellites, Polymorphism, Co-dominance, Genetic analysis, PIC
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How to cite this article:

Mukesh Kumar, Aditya Pratap Acharya, Sujit Kumar, P. Gireesh Babu, A. Pavan Kumar and Aparna Chaudhari. 2018. Genetic Diversity Study of Clarias magur using Simple Sequence Repeats.Int.J.Curr.Microbiol.App.Sci. 7(3): 3681-3690. doi: https://doi.org/10.20546/ijcmas.2018.703.426