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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 3674-3680
DOI: https://doi.org/10.20546/ijcmas.2018.703.425


Prevalence of Staphylococcus aureus in Lactating Cows with Subclinical Mastitis and their Antibiogram in Organized Dairy Farm, Maharashtra, India
Mahendra Mohan Yadav*
Research cum Development Project on Cattle, Mahatma Phule Krishi Vidyapeeth, Maharashtra State-413 722, India
*Corresponding author
Abstract:

The purpose of this study was to investigate sub-clinical mastitis in organized dairy farm and prevalence of Staphylococcus aureus with its antibiogram pattern. The present study was carried out at Research Cum Development Project on Cattle, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra. A total of 1296 lactating crossbred cows (5184 quarters) were investigated for sub-clinical mastitis. Samples positive for sub-clinical mastitis were subjected for isolation and biochemical characterization. Isolates that was confirmed as S. aureus were subjected for antibiotic-susceptibility testing. The overall quarter wise incidence of sub clinical mastitis among 1296 crossbred cows was 3.62 per cent (188 quarters out of 5184 quarters). After biochemical characterization, S. aureus was confirmed in 152 samples (80.85 %). Antibiotic resistance pattern revealed that the highest resistance to the antimicrobials used was to observed in Cephalexin (86.84%), followed by Co-trimoxazole (82.89%), Penicillin G (82.23%), Ampicillin (81.57%), Cefoperazone (76.97%), Ceftriaxone (69.73%), Cefotaxime (65.13), Amoxyclav (63.15%), and Tetracycline (54.60 %) Chloramphenicol (46.71%), Ciprifloxacin (42.10%), Gentamicin (38.81%), Enrofloxacin and Levofloxacin (19.73 % each). This study confirms the importance of S. aureus as a major mastitis causing bacterium and existence of alarming level of resistance to frequently used antibiotic by S. aureus and a potential risk for human health from nearly possible transmission of the S. aureus strains as milk borne pathogen.


Keywords: Cattle, Sub clinical mastitis, Prevalence, Organized dairy farm, Antibiogram
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How to cite this article:

Mahendra Mohan Yadav. 2018. Prevalence of Staphylococcus aureus in Lactating Cows with Subclinical Mastitis and their Antibiogram in Organized Dairy Farm, Maharashtra, India.Int.J.Curr.Microbiol.App.Sci. 7(3): 3674-3680. doi: https://doi.org/10.20546/ijcmas.2018.703.425