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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 863-874
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.099


Screening, Production and Industrial Application of Protease Enzyme From Marine Bacteria
Hala Ezzat Abou El-Hassayeb1* and Samaa M.Z. Abdel Aziz2
1Department of Marine Microbiology, National Institute of Oceanography and
Fisheries, Alexandria, Egypt
2Head of Industrial Wastewater Research, Department, ASDCO, Egypt
*Corresponding author
Abstract:

Marine sediment samples were collected from Eastern harbor of Alexandria, Egypt. All bacterial isolates were screened for protease producing microbes. Only one isolate showed maximum proteolytic activity.  The isolate was performed and identified as Bacillus subtilis. The identification process was carried out according to Bergey’s Manual of Determinative Bacteriology. The strain has the ability to tolerate 7%Nacl concentration. The amount of protease produced was expressed in microgram of tyrosine released under standard assay conditions. The total protein content of crude enzyme extracts of Bacillus subtilis was quantified which revealed 20.1mg/ml of protein content. The proteolytic bacterium gave an optimum performance was the strain exhibited the enzyme stable at pH7. In the present study Bacillus subtilis showed a remarkable activity at 40ºC. Studies pertaining to carbon sources starch were utilized by Bacillus subtilis and maximum production was achieved. Among the different nitrogen sources tested yeast extract induced maximum proteolytic activity. The crude enzyme was efficient to remove biological dyes such as crystal violet and saffranin and blood stain by Bacillus subtilis.


Keywords: Bacillus subtilis, protease,blood stain, detergent, dye, stain removal, temperature
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How to cite this article:

Hala Ezzat Abou El-Hassayeb and Samaa M.Z. Abdel Aziz. 2016. Screening, Production and Industrial Application of Protease Enzyme From Marine Bacteria.Int.J.Curr.Microbiol.App.Sci. 5(7): 863-874. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.099