National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Marine sediment samples were collected from Eastern harbor of Alexandria, Egypt. All bacterial isolates were screened for protease producing microbes. Only one isolate showed maximum proteolytic activity. The isolate was performed and identified as Bacillus subtilis. The identification process was carried out according to Bergey’s Manual of Determinative Bacteriology. The strain has the ability to tolerate 7%Nacl concentration. The amount of protease produced was expressed in microgram of tyrosine released under standard assay conditions. The total protein content of crude enzyme extracts of Bacillus subtilis was quantified which revealed 20.1mg/ml of protein content. The proteolytic bacterium gave an optimum performance was the strain exhibited the enzyme stable at pH7. In the present study Bacillus subtilis showed a remarkable activity at 40ºC. Studies pertaining to carbon sources starch were utilized by Bacillus subtilis and maximum production was achieved. Among the different nitrogen sources tested yeast extract induced maximum proteolytic activity. The crude enzyme was efficient to remove biological dyes such as crystal violet and saffranin and blood stain by Bacillus subtilis.
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